Vascularisation is not necessary for gut colonisation by enteric neural crest cells.
Vascularisation is not necessary for gut colonisation by enteric neural crest cells.
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DOI：
10.1016/j.ydbio.2013.11.007
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发表时间：
2014-01-15
影响因子：
2.7
通讯作者：
Burns, Alan J.
中科院分区：
文献类型：
--
作者：
Delalande, Jean-Marie;Natarajan, Dipa;Vernay, Bertrand;Finlay, Malcolm;Ruhrberg, Christiana;Thapar, Nikhil;Burns, Alan J.
血管系统和神经系统在它们的网络化、树状结构以及它们在成熟器官中叠加的方式上有着惊人的相似之处。先前已经提出，肠微血管系统网络引导肠神经嵴细胞（ENCC）沿着肠迁移以促进肠神经系统（ENS）的形成。为了研究迁移性ENCC、ENS形成和肠道血管发育的相互关系，我们将ENCC的命运映射与免疫标记和血管内染料注射相结合，以可视化新生血管网络。我们发现，肠和血管网络最初有非常不同的发展模式。在前肠中，ENCC通过缺乏已建立的血管网络的区域迁移。在血管丰富的区域，如中肠和后肠，迁移ENCC的分布不支持这些细胞遵循预先建立的血管网络的想法。此外，当肠道血管发育受损时，无论是在Vegfa 120/120或Tie 2-Cre; Nrp 1fl/−小鼠中遗传还是使用ENS发育的体外Wnt 1-Cre; Rosa 26 Yfp/+小鼠模型，ENCC仍然定殖于整个肠道，包括末端后肠。这些结果表明，血管网络是不必要的，以指导迁移ENCC在ENS的发展。相反，在后肠缺乏ENS的miRet 51小鼠中，该区域的血管网络似乎是正常的，这表明在早期发育中，两个网络相互独立地形成。肠道血管系统被认为是指导肠神经嵴细胞（ENCC）迁移的。我们可视化了ENCC和发育中的胃肠道血管。肠道和血管网络最初几乎没有相关性。当体内或体外血管发育受损时，ENCC定殖于肠道。因此，在ENS形成过程中，血管不需要引导ENCC迁移。
The vasculature and nervous system share striking similarities in their networked, tree-like architecture and in the way they are super-imposed in mature organs. It has previously been suggested that the intestinal microvasculature network directs the migration of enteric neural crest cells (ENCC) along the gut to promote the formation of the enteric nervous system (ENS). To investigate the inter-relationship of migrating ENCC, ENS formation and gut vascular development we combined fate-mapping of ENCC with immunolabelling and intravascular dye injection to visualise nascent blood vessel networks. We found that the enteric and vascular networks initially had very distinct patterns of development. In the foregut, ENCC migrated through areas devoid of established vascular networks. In vessel-rich areas, such as the midgut and hindgut, the distribution of migrating ENCC did not support the idea that these cells followed a pre-established vascular network. Moreover, when gut vascular development was impaired, either genetically in Vegfa120/120 or Tie2-Cre;Nrp1fl/− mice or using an in vitro Wnt1-Cre;Rosa26Yfp/+ mouse model of ENS development, ENCC still colonised the entire length of the gut, including the terminal hindgut. These results demonstrate that blood vessel networks are not necessary to guide migrating ENCC during ENS development. Conversely, in miRet51 mice, which lack ENS in the hindgut, the vascular network in this region appeared to be normal suggesting that in early development both networks form independently of each other. The gut vasculature is thought to direct migrating enteric neural crest cells (ENCC). We visualised ENCC and the vasculature in the developing gastrointestinal tract. Enteric and vascular networks initially had little correlation. ENCC colonised the gut when vascular development was impaired in vivo or in vitro. Blood vessels are thus not necessary to guide migrating ENCC during ENS formation.
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影响因子：
2.4
作者：
Freem, Lucy J.;Escot, Sophie;Burns, Alan J.
通讯作者：
Burns, Alan J.
影响因子：
2.7
作者：
Broders-Bondon, Florence;Paul-Gilloteaux, Perrine;Dufour, Sylvie
通讯作者：
Dufour, Sylvie
影响因子：
0.7
作者：
Freem, Lucy J.;Delalande, Jean Marie;Burns, Alan J.
通讯作者：
Burns, Alan J.
在肠道中建立大脑：肠神经系统的发展。
影响因子：
3.5
作者：
Goldstein AM;Hofstra RM;Burns AJ
通讯作者：
Burns AJ
影响因子：
8.5
作者：
Bou-Gharios, G;Ponticos, M;Abraham, D
通讯作者：
Abraham, D