Molecular subtypes of osteosarcoma classified by cancer stem cell related genes define immunological cell infiltration and patient survival.
Molecular subtypes of osteosarcoma classified by cancer stem cell related genes define immunological cell infiltration and patient survival.
复制标题
由肿瘤干细胞相关基因分类的骨肉瘤分子亚型决定了免疫细胞浸润和患者生存。
DOI：
10.3389/fimmu.2022.986785
复制
发表时间：
2022
影响因子：
7.3
通讯作者：
Wang, Boyang
中科院分区：
文献类型：
--
作者：
Guo, Lei;Yan, Taiqiang;Guo, Wei;Niu, Jianfang;Wang, Wei;Ren, Tingting;Huang, Yi;Xu, Jiuhui;Wang, Boyang
近年来的研究表明，肿瘤干细胞性在肿瘤发生和肿瘤进展中具有重要的生物学意义。然而，由多种肿瘤干细胞相关基因的联合作用介导的骨肉瘤中TME免疫浸润的特征仍然未知。在这项研究中，我们确定了不同的癌症干细胞相关的亚型在骨肉瘤的基础上，25个癌症干细胞相关基因的共识聚类分析，我们全面评估这些亚型之间的关联和免疫细胞浸润的TME。构建癌症干细胞（CSC）评分以量化个体肿瘤的干性。我们对218例骨肉瘤患者进行了基于25个肿瘤干细胞相关基因的综合评估。三种不同的癌症干细胞相关亚型被鉴定，它们与不同的生物学过程和临床结果相关。三种亚型具有不同的TME细胞浸润特征，CSC簇A比CSC簇B和C具有更高水平的免疫细胞浸润。我们构建了一个评分系统，称为CSC评分，以评估个体患者的干性。结果发现CSC评分对患者的预后有一定的预测作用，CSC评分低的患者生存期延长。进一步分析表明，低CSC评分与增强的免疫浸润相关。CSC评分可以预测免疫治疗的效果，低CSC评分的患者可能具有更好的免疫应答和临床预后。本研究表明，骨肉瘤中可能存在三种肿瘤干细胞相关亚型，它们与不同的患者预后和TME免疫浸润特征相关。CSC评分可用于评估个体患者的干性，提高我们对TME特征的理解，并指导更有效的免疫治疗。
Recent studies have shown that tumor stemness has biological significance in tumorigenicity and tumor progression. However, the characteristics of TME immune infiltration in osteosarcoma mediated by the combined effects of multiple cancer stem cell-related genes remain unknown. In this study, we identified different cancer stem cell-associated subtypes in osteosarcoma based on 25 cancer stem cell-associated genes by consensus clustering analysis, and we comprehensively evaluated the association between these subtypes and immunocytes infiltration in the TME. The cancer stem cell (CSC) score was constructed to quantify the stemness of individual tumors. We performed a comprehensive evaluation of 218 osteosarcoma patients based on 25 cancer stem cell-related genes. Three different cancer stem cells related subtypes were identified, which were related to different biological processes and clinical outcomes. The three subtypes have different TME cells infiltrating characteristics, and CSC Cluster A had a higher level of immunocyte infiltration compared to CSC Cluster B and C. We constructed a scoring system, called the CSC score, to assess the stemness of individual patients. Then we found that the prognosis of patients was predicted by CSC score, and patients with low CSC score had prolonged survival. Further analyses showed that low CSC score was correlated with enhanced immune infiltration. CSC score may predict the effect of immunotherapy, and patients with low CSC score may have better immune response and clinical prognosis. This study demonstrates that there could be three cancer stem cell-associated subtypes in osteosarcoma and that they were associated with different patient prognosis and TME immune infiltration characteristics. CSC score could be used to assess the stemness of individual patients, improve our comprehension of TME characteristics, and direct more effective immune therapy.
登录
查看更多内容
DOI：
10.1126/science.aac9935
发表时间：
2016-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Casey SC;Tong L;Li Y;Do R;Walz S;Fitzgerald KN;Gouw AM;Baylot V;Gütgemann I;Eilers M;Felsher DW
通讯作者：
Felsher DW
影响因子：
11.2
作者：
Gomez KE;Wu F;Keysar SB;Morton JJ;Miller B;Chimed TS;Le PN;Nieto C;Chowdhury FN;Tyagi A;Lyons TR;Young CD;Zhou H;Somerset HL;Wang XJ;Jimeno A
通讯作者：
Jimeno A
影响因子：
11.2
作者：
Collins, AT;Berry, PA;Maitland, NJ
通讯作者：
Maitland, NJ
影响因子：
7.7
作者：
Galardi, Silvia;Savino, Mauro;Nasi, Sergio
通讯作者：
Nasi, Sergio
影响因子：
78.5
作者：
Bjerkvig, R;Tysnes, BB;Terzis, AJA
通讯作者：
Terzis, AJA