Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions.
Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions.
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独立的扭结纳米线晶体管探针在三个维度上针对靶向细胞内记录。
DOI：
10.1038/nnano.2013.273
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发表时间：
2014-02
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
--
作者：
记录细胞内的生物电信号对于理解神经和心脏系统中细胞和细胞网络的基本行为是至关重要的。作为细胞内记录的标准工具，膜片钳微吸管得到了广泛的应用，但在减小尖端尺寸、重复使用吸管的能力以及与细胞质的离子交换方面仍然有限。最近的努力是开发基于芯片的新工具，包括微米到纳米级的金属柱、基于晶体管的扭结纳米线和纳米管设备。这些纳米尺度的工具对基于芯片的多路传输很感兴趣，但到目前为止，它们排除了从特定细胞区域和/或亚细胞结构进行定向记录的可能性。在这里，我们通过制造具有编码场效应晶体管探测器的扭结的硅纳米线作为尖端，以一般的方式克服这一限制。这些探针可以在标准显微镜内的三维(3D)中操作，以瞄准特定的细胞/细胞区域，并记录来自不同目标细胞的稳定的全幅细胞内动作电位，而不需要清洁或更换尖端。用独立的纳米线和膜片钳探针对同一细胞进行的同时测量表明，在没有对原始纳米线信号进行校正的情况下，记录了相同的动作电位、幅度和时间特性。此外，我们还展示了对不同离子通道阻滞剂应用于细胞时动作电位变化的实时监测，并通过两个独立的纳米线探针的独立操作从细胞进行多路复用记录。
Recording intracellular bioelectrical signals is central to understanding the fundamental behaviour of cells and cell-networks in, for example, neural and cardiac systems. The standard tool for intracellular recording, the patch-clamp micropipette is widely applied, yet remains limited in terms of reducing the tip size, the ability to reuse the pipette, and ion exchange with the cytoplasm. Recent efforts have been directed towards developing new chip-based tools, including micro-to-nanoscale metal pillars, transistor-based kinked nanowire and nanotube devices. These nanoscale tools are interesting with respect to chip-based multiplexing, but, to date, preclude targeted recording from specific cell regions and/or subcellular structures. Here we overcome this limitation in a general manner by fabricating free-standing probes where a kinked silicon nanowire with encoded field-effect transistor detector serves as the tip end. These probes can be manipulated in three dimensions (3D) within a standard microscope to target specific cells/cell regions, and record stable full-amplitude intracellular action potentials from different targeted cells without the need to clean or change the tip. Simultaneous measurements from the same cell made with free-standing nanowire and patch-clamp probes show that the same action potential amplitude and temporal properties are recorded without corrections to the raw nanowire signal. In addition, we demonstrate real-time monitoring of changes in the action potential as different ion-channel blockers are applied to cells, and multiplexed recording from cells by independent manipulation of two free-standing nanowire probes.
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纳米电子生物学领域：从纳米探针进行活细胞中的动作潜在记录到三维机器人组织。
影响因子：
17.4
作者：
Duan X;Fu TM;Liu J;Lieber CM
通讯作者：
Lieber CM
影响因子：
38.3
作者：
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影响因子：
10.8
作者：
Gao R;Strehle S;Tian B;Cohen-Karni T;Xie P;Duan X;Qing Q;Lieber CM
通讯作者：
Lieber CM
影响因子：
3.4
作者：
CLARK, BA;MOBBS, P
通讯作者：
MOBBS, P
影响因子：
20.1
作者：
AKBARALI, HI;WYSE, DG;GILES, WR
通讯作者：
GILES, WR