Outside looking in: nanotube transistor intracellular sensors.
Outside looking in: nanotube transistor intracellular sensors.
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DOI：
10.1021/nl301623p
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发表时间：
2012-06-13
期刊：
影响因子：
10.8
通讯作者：
Lieber CM
中科院分区：
文献类型：
--
作者：
Gao R;Strehle S;Tian B;Cohen-Karni T;Xie P;Duan X;Qing Q;Lieber CM
基于纳米线的场效应晶体管（NWFET），包括具有平面和三维（3D）配置的器件，由于其小尺寸和高灵敏度而被积极探索作为用于细胞外和细胞内记录的检测器。在这里，我们报告了一种新的针形纳米探针的合成，制造和表征的基础上，有源硅纳米管晶体管，ANTT，使高分辨率的细胞内记录。在ANTT探针中，与硅纳米管的源极/漏极（S/D）接触在一端制造，从外部溶液钝化，然后可以通过填充管的溶液从相对的纳米管端记录电势的时间依赖性变化。在水溶液中的电导与水门电位的测量表明，ANTT探针选择性地门控由纳米管内的电位变化，从而展示了ANTT设备的基本操作原理。研究接口的ANTT探针与自发跳动的心肌细胞产生稳定的细胞内动作电位类似于其他电生理技术的报告。此外，利用ANTT装置的直接制造来在单个探针的末端制备多个ANTT结构，这使得能够多路记录来自单个细胞的细胞内动作电位，以及单个ANTT装置探针的多路阵列。这些研究为通过细胞网络从单个细胞进行多位点记录开辟了独特的机会。
Nanowire-based field-effect transistors (NWFETs), including devices with planar and three-dimensional (3D) configurations, are being actively explored as detectors for extra- and intracellular recording due to their small size and high sensitivities. Here we report the synthesis, fabrication and characterization of a new needle-shaped nanoprobe based on an active silicon nanotube transistor, ANTT, that enables high-resolution intracellular recording. In the ANTT probe, the source/drain (S/D) contacts to the silicon nanotube are fabricated on one end, passivated from external solution, and then time-dependent changes in potential can be recorded from the opposite nanotube end via the solution filling the tube. Measurements of conductance versus water-gate potential in aqueous solution show that the ANTT probe is selectively gated by potential changes within the nanotube, thus demonstrating the basic operating principle of the ANTT device. Studies interfacing the ANTT probe with spontaneously beating cardiomyocytes yielded stable intracellular action potentials similar to those reported by other electrophysiological techniques. In addition, the straightforward fabrication of ANTT devices was exploited to prepare multiple ANTT structures at the end of single probes, which enabled multiplexed recording of intracellular action potentials from single cells, and multiplexed arrays of single ANTT device probes. These studies open up unique opportunities for multisite recordings from individual cells through cellular networks.
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