Wide host ranges of herbivorous beetles? Insights from DNA bar coding.
Wide host ranges of herbivorous beetles? Insights from DNA bar coding.
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DOI：
10.1371/journal.pone.0074426
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ito M
中科院分区：
文献类型：
--
作者：
Kishimoto-Yamada K;Kamiya K;Meleng P;Diway B;Kaliang H;Chong L;Itioka T;Sakai S;Ito M
很少有研究研究热带食草性昆虫的寄主专一性。事实上，东南亚热带雨林中食草性昆虫的大部分营养相互作用仍然未知，也没有确定在这个生态系统的草食动物中是否普遍存在多食性摄食。本研究使用DNA条形码来揭示婆罗洲热带雨林中成虫咀嚼树叶的营养联系。从昆虫体内提取成虫摄取的植物材料，然后从该材料中扩增出叶绿体rbcL序列的一部分。利用现有的叶绿体DNA参考数据库，在科的水平上鉴定了这些植物。我们对11种金龟子的食性分析成功地确定了它们的寄主植物家族，结果表明，5种甲虫取食被子植物中两个以上的科，4种取食裸子植物和/或蕨类的几个科，以及多个被子植物科。这些发现表明，与生态和分类上相距较远的植物有关的多面性金龟子构成了婆罗洲热带雨林植物-昆虫网络的一部分。
There are very few studies that have investigated host-specificity among tropical herbivorous insects. Indeed, most of the trophic interactions of herbivorous insects in Southeast Asian tropical rainforests remain unknown, and whether polyphagous feeding is common in the herbivores of this ecosystem has not been determined. The present study employed DNA bar coding to reveal the trophic associations of adult leaf-chewing chrysomelid beetles in a Bornean rainforest. Plant material ingested by the adults was retrieved from the bodies of the insects, and a portion of the chloroplast rbcL sequence was then amplified from this material. The plants were identified at the family level using an existing reference database of chloroplast DNA. Our DNA-based diet analysis of eleven chrysomelid species successfully identified their host plant families and indicated that five beetle species fed on more than two families within the angiosperms, and four species fed on several families of gymnosperms and/or ferns together with multiple angiosperm families. These findings suggest that generalist chrysomelid beetles associated with ecologically and taxonomically distant plants constitute a part of the plant-insect network of the Bornean rainforest.
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影响因子：
2.9
作者：
APPANAH, S
通讯作者：
APPANAH, S
影响因子：
64.8
作者：
COLEY, PD
通讯作者：
COLEY, PD
影响因子：
1.9
作者：
GENTRY, AH
通讯作者：
GENTRY, AH
影响因子：
4.8
作者：
Barone, JA
通讯作者：
Barone, JA
DOI：
10.1073/pnas.0812297106
发表时间：
2009-02-24
影响因子：
11.1
作者：
Awano, Tomoyuki;Johnson, Gary S.;Coates, Joan R.
通讯作者：
Coates, Joan R.