Rab32 is important for autophagy and lipid storage in Drosophila.
Rab32 is important for autophagy and lipid storage in Drosophila.
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Rab32 对于果蝇自噬和脂质储存很重要
DOI：
10.1371/journal.pone.0032086
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Wang C;Liu Z;Huang X
脂质是所有生物体的重要组成部分。在细胞内，脂质主要储存在一种特定类型的细胞器中，称为脂滴。控制脂滴动力学的分子机制很少被探索。大量蛋白质组学研究已对脂滴的蛋白质组成进行了分析，并鉴定了大量脂滴相关蛋白，包括 Rab 小 GTP 酶。 Rab 蛋白已知参与许多细胞内膜事件；然而，它们在脂滴中的确切作用很大程度上尚未被探索。在这里，我们系统地研究了果蝇 Rab 家族蛋白在幼虫脂肪组织（脂肪体）脂质储存中的作用。研究发现 Rab32 和其他几种 Rab 会影响脂滴的大小以及脂质水平。进一步的研究表明 Rab32 和 Rab32 GEF/Claret 可能参与自噬，从而影响脂质储存。自噬途径中几个成分的功能丧失突变体会对脂质储存产生类似的影响。这些结果凸显了 Rabs 在调节脂质代谢方面的潜在功能。
Lipids are essential components of all organisms. Within cells, lipids are mainly stored in a specific type of organelle, called the lipid droplet. The molecular mechanisms governing the dynamics of lipid droplets have been little explored. The protein composition of lipid droplets has been analyzed in numerous proteomic studies, and a large number of lipid droplet-associated proteins have been identified, including Rab small GTPases. Rab proteins are known to participate in many intracellular membranous events; however, their exact role in lipid droplets is largely unexplored. Here we systematically investigate the roles of Drosophila Rab family proteins in lipid storage in the larval adipose tissue, fat body. Rab32 and several other Rabs were found to affect the size of lipid droplets as well as lipid levels. Further studies showed that Rab32 and Rab32 GEF/Claret may be involved in autophagy, consequently affecting lipid storage. Loss-of-function mutants of several components in the autophagy pathway result in similar effects on lipid storage. These results highlight the potential functions of Rabs in regulating lipid metabolism.
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