Identification of novel yolk ferritins unique to planarians: planarians supply aluminum rather than iron to vitellaria in egg capsules
Identification of novel yolk ferritins unique to planarians: planarians supply aluminum rather than iron to vitellaria in egg capsules
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涡虫特有的新型卵黄铁蛋白的鉴定：涡虫向卵囊中的黄藻提供铝而不是铁
DOI：
10.1007/s00441-021-03506-8
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发表时间：
2021
影响因子：
3.6
通讯作者：
Kobayashi
中科院分区：
文献类型：
--
作者：
Kawase Osamu;Iwaya Hisashi;Asano Yoshiya;Inoue Hiromoto;Kudo Seiya;Sasahira Motoki;Azuma Nobuyuki;Kondoh Daisuke;Ichikawa-Seki Madoka;Xuan Xuenan;Sakamoto Kimitoshi;Okamoto Hikaru;Nakadate Hinaki;Inoue Wataru;Saito Ikuma;Narita Miyu;Sekii Kiyono;Kobayashi
除扁形动物外，所有动物都产含有蛋黄的蛋，称为“内黄”蛋。然而，在扁形动物门中，只有Neoophora产生“外黄”卵（卵囊），其中蛋黄储存在卵母细胞周围的卵黄细胞中。卵黄细胞来源于卵黄腺。卵黄是重要的生殖器官，可以通过研究来阐明扁形动物在进化上保守的独特机制。目前，只有有限的分子水平的信息是可用的卵黄。目前的研究确定了主要的卵黄特异性蛋白在淡水真涡虫，琉球三角洲，使用肽质量指纹（PMF）和表达分析。氨基酸序列分析和同源性分析表明，所确定的主要的卵黄特异性的新的卵黄铁蛋白在真涡虫（Tricladida）中是保守的。由于铁蛋白在铁（铁）储存中发挥着重要作用，因此我们使用非血红素铁组织化学、基于电感耦合等离子体质谱和透射电子显微镜的元素分析来检查卵黄和外黄卵中含有的金属元素-能量分散X射线光谱分析。有趣的是，卵黄和鸡蛋胶囊含有大量的铝（Al），但没有铁。卵黄铁蛋白基因的敲低导致了卵囊体积的减少，幼鱼的异常，以及卵黄中铝含量的增加。D.卵黄铁蛋白因此，琉球藻可能通过其汇集铝的功能来调节卵黄中铝的浓度，从而保护卵黄囊的生产和胚胎的正常发育。
All animals, other than Platyhelminthes, produce eggs containing yolk, referred to as “entolecithal” eggs. However, only Neoophora, in the phylum Platyhelminthes, produce “ectolecithal” eggs (egg capsules), in which yolk is stored in the vitelline cells surrounding oocytes. Vitelline cells are derived from vitellaria (yolk glands). Vitellaria are important reproductive organs that may be studied to elucidate unique mechanisms that have been evolutionarily conserved within Platyhelminthes. Currently, only limited molecular level information is available on vitellaria. The current study identified major vitellaria-specific proteins in a freshwater planarian,Dugesia ryukyuensis, using peptide mass fingerprinting (PMF) and expression analyses. Amino acid sequence analysis and orthology analysis via OrthoFinder ver.2.3.8 indicated that the identified major vitellaria-specific novel yolk ferritins were conserved in planarians (Tricladida). Because ferritins play an important role in Fe (iron) storage, we examined the metal elements contained in vitellaria and ectolecithal eggs, using non-heme iron histochemistry, elemental analysis based on inductively coupled plasma mass spectrometry and transmission electron microscopy– energy-dispersive X-ray spectroscopy analysis. Interestingly, vitellaria and egg capsules contained large amounts of aluminum (Al), but not Fe. The knockdown of the yolk ferritin genes caused a decrease in the volume of egg capsules, abnormality in juveniles, and increase in Al content in vitellaria. Yolk ferritins ofD. ryukyuensismay regulate Al concentration in vitellaria via their pooling function of Al and protect the egg capsule production and normal embryogenesis from Al toxicity.
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影响因子：
2.6
作者：
GREMIGNI, V;FALLENI, A
通讯作者：
FALLENI, A
淡水涡虫早期胚胎发育过程中合胞体形成的电子显微镜研究
影响因子：
2.6
作者：
T. Sakurai
通讯作者：
T. Sakurai
影响因子：
3.9
作者：
M. Nagaoka;T. Maitani
通讯作者：
M. Nagaoka;T. Maitani
影响因子：
0.9
作者：
Kobayashi, K;Koyanagi, R;Hoshi, M
通讯作者：
Hoshi, M
影响因子：
2.6
作者：
T. Maezawa;Hiroyuki Tanaka;Haruka Nakagawa;Mizuki Ono;Manabu Aoki;M. Matsumoto;T. Ishida;K. Horiike;K. Kobayashi
通讯作者：
T. Maezawa;Hiroyuki Tanaka;Haruka Nakagawa;Mizuki Ono;Manabu Aoki;M. Matsumoto;T. Ishida;K. Horiike;K. Kobayashi