Motion compensation in structured illumination fluorescence endomicroscopy
Motion compensation in structured illumination fluorescence endomicroscopy
复制标题
结构照明荧光内窥镜中的运动补偿
DOI：
10.1117/12.2509590
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Thrapp A
中科院分区：
文献类型：
--
作者：
Thrapp A
内窥镜检查是一种获得体内实时图像的技术，无需对组织样本进行活检。可以使用光纤束、相机、LED 和滤光片构建简单的荧光内窥镜，并且当探头在组织上移动时可以对各个图像进行镶嵌以增加图像尺寸。然而，为了提高图像对比度，需要光学切片来去除返回的离焦光。通常，这是使用共焦技术来完成的，需要更昂贵的激光源和机械扫描镜，这限制了帧速率。结构照明显微镜 (SIM) 使用投影到样品上的线条图案来进行计算光学切片。这消除了点扫描的需要，并允许使用非相干光源，例如 LED，但代价是信噪比有所损失。然而，由于 SIM 需要组合多个图像，探头的运动会导致严重的图像伪影，从而阻碍了镶嵌技术的使用。我们报告了一种 SIM 内窥镜，它使用数字微镜器件 (DMD) 高速生成线条图案，并且能够动态更改图案。与高速摄像机相结合，这可以显着减少运动伪影，但不足以允许视频镶嵌技术。因此，我们通过将照明图案定向为与运动方向平行并执行帧间配准和校正来进一步减少伪影。这为低成本、多功能、光学切片内窥镜检查提供了潜力。
Endomicroscopy is a technique for obtaining real-time images in vivo, eliminating the need to biopsy a tissue sample. A simple fluorescence endomicroscope can be constructed using a fiber bundle, camera, LED and filters, and individual images can be mosaicked as the probe is moved across the tissue to increase the image size. However, to improve image contrast optical sectioning is required for the removal of returning out-of-focus light. Commonly, this is done using the confocal technique, requiring more expensive laser sources and mechanical scanning mirrors which limits the frame rate. Structured illumination microscopy (SIM) instead uses line patterns projected onto the sample to allow for computational optical sectioning. This eliminates the need for point scanning and allows an incoherent light source, such as an LED, to be used, at the cost of some loss of signal-to-noise ratio. However, as SIM requires multiple images to be combined, motion of the probe results in severe image artefacts, preventing the use of mosaicking techniques. We report a SIM endomicroscope using a digital micro-mirror device (DMD) to generate line patterns at high speed, and with the ability to change the patterns on the fly. Combined with a high-speed camera, this reduces motion artefacts significantly, but not sufficiently to allow for video mosaicking techniques. We therefore demonstrate further reduction of artefacts by orienting the illumination patterns parallel to the direction of motion and performing inter-frame registration and correction. This offers potential for low cost, versatile, optically-sectioned endomicroscopy.
影响因子：
3.8
作者：
Bozinovic, Nenad;Ventalon, Cathie;Mertz, Jerome
通讯作者：
Mertz, Jerome
随机比较 3 种不同尺寸的活检钳对巴雷特食管取样质量的影响。
影响因子：
7.7
作者：
Gonzalez,Susana;Yu,WoojinM;Smith,MichaelS;Slack,KristenN;Rotterdam,Heidrun;Abrams,JulianA;Lightdale,CharlesJ
通讯作者：
Lightdale,CharlesJ