Age related changes of rib cortical bone matrix and the application to forensic age-at-death estimation.
Age related changes of rib cortical bone matrix and the application to forensic age-at-death estimation.
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DOI：
10.1038/s41598-021-81342-0
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发表时间：
2021-01-22
影响因子：
4.6
通讯作者：
Kranioti EF
中科院分区：
文献类型：
--
作者：
Bonicelli A;Zioupos P;Arnold E;Rogers KD;Xhemali B;Kranioti EF
法医人类学包括，除其他应用外，积极识别未知的人类骨骼遗骸。这一过程的第一步是评估生物特征，即：性别、年龄、身高和血统。在法医学背景下，年龄估计是鉴定过程中的主要挑战之一。最近建立的受理标准正在推动研究人员对标准化的方法程序。尽管有这些变化，经验仍然在人类学研究中发挥着核心作用。为了避免这个问题，年龄估计程序（i）必须提交给科学界并在同行评审的期刊上发表，（ii）准确解释程序，（iii）提供关于估计准确性和可能错误率的明确信息。为了满足所有这些要求，已经开发了许多基于生理过程的方法，这些方法在分子水平上导致各种组织结构的生化变化，例如DNA甲基化和端粒缩短的修饰，蛋白质的外消旋化和稳定同位素分析。目前的工作提出了一种新的系统性年龄估计方法，该方法基于追踪肋骨皮质骨基质的物理化学和机械变性。本研究使用了113个肋骨标本的尸检材料。通过标准生物机械（纳米压痕和微米压痕）、物理（TGA/DSC、XRD和FTIR）和组织形态测量（孔隙率-ImageJ）方法测量一组33个参数。逐步回归被用来创建方程，以产生最好的“死亡年龄估计”与尸体的真实的年龄。五个方程，在最好的情况下，方程计数7个参数的R2 = 0.863和平均绝对误差为4.64年。本方法满足所有的受理标准先前描述的。此外，该方法是经验独立的，因此可以在没有法医人类学和人体解剖学的先前专业知识的情况下进行。
Forensic anthropology includes, amongst other applications, the positive identification of unknown human skeletal remains. The first step in this process is an assessment of the biological profile, that is: sex, age, stature and ancestry. In forensic contexts, age estimation is one of the main challenges in the process of identification. Recently established admissibility criteria are driving researchers towards standardisation of methodological procedures. Despite these changes, experience still plays a central role in anthropological examinations. In order to avoid this issue, age estimation procedures (i) must be presented to the scientific community and published in peer reviewed journals, (ii) accurately explained in terms of procedure and (iii) present clear information about the accuracy of the estimation and possible error rates. In order to fulfil all these requirements, a number of methods based on physiological processes which result in biochemical changes in various tissue structures at the molecular level, such as modifications in DNA-methylation and telomere shortening, racemization of proteins and stable isotopes analysis, have been developed. The current work proposes a new systematic approach in age estimation based on tracing physicochemical and mechanical degeneration of the rib cortical bone matrix. This study used autopsy material from 113 rib specimens. A set of 33 parameters were measured by standard bio-mechanical (nanoindentation and microindentation), physical (TGA/DSC, XRD and FTIR) and histomorphometry (porosity-ImageJ) methods. Stepwise regressions were used to create equations that would produce the best ‘estimates of age at death’ vs real age of the cadavers. Five equations were produced; in the best of cases an equation counting 7 parameters had an R2 = 0.863 and mean absolute error of 4.64 years. The present method meets all the admissibility criteria previously described. Furthermore, the method is experience-independent and as such can be performed without previous expert knowledge of forensic anthropology and human anatomy.
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影响因子：
2.1
作者：
Ghasemi A;Zahediasl S
通讯作者：
Zahediasl S
影响因子：
2.8
作者：
KERLEY, ER
通讯作者：
KERLEY, ER
影响因子：
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作者：
Godde, Kanya;Hens, Samantha M.
通讯作者：
Hens, Samantha M.
影响因子：
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作者：
Griffin, R. C.;Moody, H.;Collins, M. J.
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Collins, M. J.
影响因子：
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作者：
HANDSCHIN, RG;STERN, WB
通讯作者：
STERN, WB