Netropsin specifically recognizes one of the two conformationally equivalent strands of poly(dA).poly(dT). One dimensional NMR study at 500 MHz involving NOE transfer between netropsin and DNA protons.
Netropsin specifically recognizes one of the two conformationally equivalent strands of poly(dA).poly(dT). One dimensional NMR study at 500 MHz involving NOE transfer between netropsin and DNA protons.
复制标题
Netropsin 特异性识别聚 (dA).聚 (dT) 的两条构象等效链之一。
DOI：
10.1080/07391102.1985.10507625
复制
发表时间：
1985
影响因子：
4.4
通讯作者：
Sarma,RH
中科院分区：
文献类型：
--
作者：
Sarma,MH;Gupta,G;Sarma,RH
最近的观察表明，在溶液中没有发现poly（dA）·poly（dT）的异主结构模型，并且在该DNA中，两条链是构象相等的(J。Biomole。DNA-netropsin复合体的结构动力学的一个新的维度。抗生素是否以某种方式区分这两条链，并特异性地只与其中一条构象相等的链相互作用？模型研究表明,netropsin可以绑定到小沟的dA-strand这样H-bonds之间形成亚氨基的质子N4-H N6-H, N8-H netropsin和N3或可以绑定到dT-strand的原子之间的小沟,形成H-bonds imino-protons N4-H, N6-H, N8-H netropsin和O2原子的t .如果netropsin结合dA-strand AH2原子聚(dA)聚(dT)将更接近亚氨基的protrons N4-H, N6-H,netropsin的N8-H和pyrorole环质子C5-H， Cll-H，如果netropsin与dt链结合。为了区分这些可能性，在药物-DNA复合物中进行了涉及netropsin和DNA质子之间NOE能量转移的实验。AH2辐照后netropsin·poly(dA)-poly（dT）配合物的NOE谱差异表明，netropsin的亚胺环和吡罗环质子上存在优势NOE。当netropsin吡咯环质子辐照时，DNA的磁化转移发生在AH2处。这些观察结果表明，netropsin结合到聚(dA)-聚（dT）的dA链上，尽管dA/dT链是构象相等的。
Recent observations that the heteronomous structural model for poly(dA)·poly(dT) is not found in solution and that in this DNA, the two strands are conformationally equivalent(J. Biomole. Str. Dyns. 2, 1057 (1985)), has added a new dimension to the structural dynamics of DNA-netropsin complex. Does the antibiotic somehow distinguish between the two strands and specifically interact with only one of the conformationally equivalent strands?Model-building studies suggest that netropsin can either bind to the dA-strand in the minor groove such that H-bonds are formed between the imino protons N4-H, N6-H, N8-H of netropsin and N3 atoms of A or can bind to the dT-strand in the minor groove and form H-bonds between the imino-protons N4-H, N6-H, N8-H of netropsin and O2 atoms of T. If netropsin binds to the dA-strand, AH2 atoms of poly(dA)-poly(dT) would be in closer proximity to the imino protrons N4-H, N6-H, N8-H and pyrrole ring protons C5-H, Cll-H of netropsin than they would be, if netropsin binds to the dT-strand. In order to distinguish these possibilities experiments were conducted which involved NOE energy transfer between netropsin and DNA protons in the drug-DNA complex. Difference NOE spectra of netropsin·poly(dA)-poly(dT) complex in which AH2 was irradiated indicate that dominant NOEs were observed at the imino and pyrrole ring protons of netropsin. When the netropsin pyrrole ring protons were irradiated, the magnetization transfer was at AH2 of DNA. These observations suggest that netropsin binds to the dA-strand of poly(dA)-poly(dT) even though dA/dT strands are conformationally equivalent.
登录
查看更多内容
netropsin 和偏端霉素 A 与 tRNA(phe) 发生意外的大沟结合。
影响因子：
4.4
作者：
J. Rubin;M. Sundaralingam
通讯作者：
M. Sundaralingam
DOI：
--
发表时间：
1979
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
D. Patel
通讯作者：
D. Patel
netropsin-poly(dA-dT).poly(dA-dT) 复合物的结构和动力学：500 MHz 1H NMR 研究。
影响因子：
4.4
作者：
Gupta,G;Sarma,MH;Sarma,RH
通讯作者：
Sarma,RH
抗生素-DNA 相互作用：溶液中的 netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) 复合物中的分子间核 Overhauser 效应。
DOI：
--
发表时间：
1982
影响因子：
11.1
作者：
D. Patel
通讯作者：
D. Patel
netropsin 与 tRNA（Phe）结合的理论探索。
影响因子：
4.4
作者：
K. Zakrzewska;B. Pullman
通讯作者：
B. Pullman