Retinal degeneration in two lines of transgenic S334ter rats.
Retinal degeneration in two lines of transgenic S334ter rats.
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DOI：
10.1016/j.exer.2010.12.001
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发表时间：
2011-03
影响因子：
3.4
通讯作者：
Cuenca, N.
中科院分区：
文献类型：
--
作者：
Martinez-Navarrete, G.;Seiler, M. J.;Aramant, R. B.;Fernandez-Sanchez, L.;Pinilla, I.;Cuenca, N.
关键词：
本研究的目的是检查视网膜突触连接的变化和凋亡的位置和速度在转基因S334 ter线3和线5感光细胞变性大鼠。在P11-13、P30、P60、P90的杂合S334 ter-line-3和line-5以及在P60的几个对照非营养不良大鼠（Long Evans和Sprague-Dawley）通过免疫组织化学对各种细胞和突触标记物以及通过PNA和TUNEL标记进行解剖学研究。S334 ter line-3表现出最快的退化速率，光感受器早期丢失，在P30时保留1-2层，并且在P60时仅保留锥细胞。5号线在P30时有4-5层剩余，在P60-90时有很少的杆剩余。在这两种细胞系中，水平细胞突起（包括树突和轴突）在P11-13时减少，在P60时外丛状层（OPL）中显示间隙，并且在P90时几乎看不到终末尖端。双极细胞树突回缩，沿OPL形成沿着簇。IPL中的A-II无长突细胞的突触终末失去了大部分的小清蛋白免疫反应性。两株细胞凋亡率不同。Line-3大鼠在P11时表现出许多受影响的光感受器，占据外核层的最内部。线-5显示在P13的相同位置内凋亡细胞的数量较低。总之，S334 ter品系3大鼠的变性进展快于品系5。在两种型号中，水平和双极端子在P11-P13处已经受到影响。细胞凋亡与突变的视紫红质转基因有关;首先受影响的感光细胞是那些暴露于接近OPL的光的细胞。
Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprague-Dawley) at P60, were studied anatomically by immunohistochemistry for various cell and synaptic markers, and by PNA and TUNEL label.- S334ter line-3 exhibited the fastest rate of degeneration with an early loss of photoreceptors, with 1–2 layers remaining at P30, and only cones left at P60. Line-5 had 4–5 layers left at P30, and very few rods left at P60-90. In both lines, horizontal cell processes (including dendrites and axon) were diminished at P11-13, showing gaps in the outer plexiform layer (OPL) at P60, and at P90, almost no terminal tips could be seen. Bipolar cells showed a retraction of their dendrites forming clusters along the OPL. Synaptic terminals of A-II amacrine cells in the IPL lost most of their parvalbumin-immunoreactivity. The apoptosis rate was different in both lines. Line-3 rats showed many photoreceptors affected at P11, occupying the innermost part of the outer nuclear layer. Line-5 showed a lower number of apoptotic cells within the same location at P13. In summary, the S334ter line-3 rat has a faster progression of degeneration than line-5. The horizontal and bipolar terminals are already affected at P11-P13 in both models. Apoptosis is related to the mutated rhodopsin transgene; the first photoreceptor cells affected are those more exposed to light close to the OPL.
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影响因子：
4
作者：
Jensen, Ralph J.;Rizzo, Joseph F., III
通讯作者：
Rizzo, Joseph F., III
影响因子：
17.8
作者：
Berger, Wolfgang;Kloeckener-Gruissem, Barbara;Neidhardt, John
通讯作者：
Neidhardt, John
影响因子：
3.3
作者：
Nagar, S.;Krishnamoorthy, V.;Dhingra, N. K.
通讯作者：
Dhingra, N. K.
影响因子：
2.5
作者：
EULER, T;WASSLE, H
通讯作者：
WASSLE, H
影响因子：
6.1
作者：
Kolomiets, Bogdan;Dubus, Elisabeth;Picaud, Serge
通讯作者：
Picaud, Serge