Human immunodeficiency virus infection of T cells and monocytes proceeds via receptor-mediated endocytosis.
Human immunodeficiency virus infection of T cells and monocytes proceeds via receptor-mediated endocytosis.
复制标题
DOI：
10.1083/jcb.107.3.959
复制
发表时间：
1988-09
期刊：
影响因子：
--
通讯作者：
Price TM
中科院分区：
文献类型：
--
作者：
Pauza CD;Price TM
32p标记的人类免疫缺陷病毒（HIV）在人T淋巴样细胞系CEM中的内化和脱包率与受体介导的内吞作用进入机制一致。电镜观察核内体内的病毒粒子证实了这一解释。经OKT4A抗体预处理后，病毒的结合和传染性均受到相同程度的抑制，因此，CD4受体依赖的内化途径可能是感染的进入途径。人单母细胞细胞系U937的内化模式更为复杂，涉及快速有效的不依赖cd4的内化。电镜显示存在大的细胞内囊泡，每个囊泡含有几个病毒粒子。针对CD4病毒受体的抗体能有效阻断感染，但不能显著降低HIV在U937细胞系中的结合或内化。因此，U937细胞具有不依赖cd4的病毒内化途径，这与感染性HIV的进入途径不一致。
The rates of internalization and uncoating of 32P-labelled human immunodeficiency virus (HIV) in the human T lymphoid cell line CEM are consonant with a receptor-mediated endocytosis mechanism of entry. This interpretation was affirmed by electron microscopic observation of virions within endosomes. Virus binding and infectivity were inhibited to the same extent by pretreatment with OKT4A antibody, therefore, the CD4 receptor-dependent pathway of internalization appears to be the infectious route of entry. The pattern of internalization by the human monoblastoid cell line U937 proved to be more complex, involving rapid and efficient CD4-independent internalization. Electron microscopy revealed the presence of large intracellular vesicles, each containing several virions. Antibody against the CD4 receptor for virus efficiently blocked infection, but did not reduce significantly HIV binding or internalization in the U937 cell line. Consequently, U937 cells have a CD4-independent pathway of virus internalization that does not coincide with the route of entry for infectious HIV.
登录
查看更多内容
影响因子：
3.8
作者：
NISHIYAMA, Y;ITO, Y;NAGATA, I
通讯作者：
NAGATA, I
影响因子：
3.7
作者：
OKADA, Y;KOSEKI, I;MATSUI, Y
通讯作者：
MATSUI, Y
影响因子：
3.7
作者：
PAUZA, CD;BLEIL, JD;LENNOX, ES
通讯作者：
LENNOX, ES
影响因子：
3.8
作者：
CHANYFOURNIER, F;CHANY, C;LAFAY, F
通讯作者：
LAFAY, F
影响因子：
56.9
作者：
MCDOUGAL, JS;KENNEDY, MS;NICHOLSON, JKA
通讯作者：
NICHOLSON, JKA