Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers.
Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers.
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DOI：
10.1262/jrd.2016-075
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Ohkura S
中科院分区：
文献类型：
--
作者：
Hassaneen A;Naniwa Y;Suetomi Y;Matsuyama S;Kimura K;Ieda N;Inoue N;Uenoyama Y;Tsukamura H;Maeda KI;Matsuda F;Ohkura S
阐明控制繁殖的生理机制是提高牛的生育能力和开发控制繁殖功能的新药剂的明显策略。本研究旨在鉴定牛下丘脑中的 Kisspeptin 神经元，阐明牛脑中也存在一个中心机制，因为已知 Kisspeptin 在刺激其他哺乳动物的促性腺激素释放激素 (GnRH)/促性腺激素分泌中发挥重要作用。为了表征牛下丘脑中的 Kisspeptin 神经元，检查了 Kisspeptin 和神经激肽 B (NKB) 或 Kisspeptin 和强啡肽 A (Dyn) 的共定位。下丘脑组织是在卵泡期和黄体期从日本黑牛或日本黑牛×荷斯坦杂交牛身上收集的。脑切片，包括弓状核 (ARC) 和视前区 (POA)，用 Kisspeptin 和 NKB 或 Dyn 进行双重免疫染色。在 ARC 中，NKB 和 Dyn 在卵泡期和黄体期均共定位于 Kisspeptin 神经元中，这表明奶牛体内存在含有 Kisspeptin/NKB/Dyn 的神经元（称为 KNDy 神经元）。在 POA 中，未检测到 Kisspeptin 与 NKB 或 Dyn 的共定位。 Kisspeptin在卵泡期的表达高于黄体期，表明奶牛POA中Kisspeptin的表达受到雌激素的正向控制。 ARC 和 POA 中的 Kisspeptin 神经元群可能分别在调节奶牛 GnRH 脉冲和激增中发挥重要作用。
Elucidating the physiological mechanisms that control reproduction is an obvious strategy for improving the fertility of cattle and developing new agents to control reproductive functions. The present study aimed to identify kisspeptin neurons in the bovine hypothalamus, clarifying that a central mechanism is also present in the cattle brain, as kisspeptin is known to play an important role in the stimulation of gonadotropin-releasing hormone (GnRH)/gonadotropin secretion in other mammals. To characterize kisspeptin neurons in the bovine hypothalamus, the co-localizations of kisspeptin and neurokinin B (NKB) or kisspeptin and dynorphin A (Dyn) were examined. Hypothalamic tissue was collected from Japanese Black or Japanese Black × Holstein crossbred cows during the follicular and luteal phases. Brain sections, including the arcuate nucleus (ARC) and the preoptic area (POA), were dual immunostained with kisspeptin and either NKB or Dyn. In the ARC, both NKB and Dyn were co-localized in kisspeptin neurons during both the follicular and luteal phases, demonstrating the presence of kisspeptin/NKB/Dyn-containing neurons, referred to as KNDy neurons, in cows. In the POA, no co-localization of kisspeptin with either NKB or Dyn was detected. Kisspeptin expression in the follicular phase was higher than that in the luteal phase, suggesting that kisspeptin expression in the POA is positively controlled by estrogen in cows. The kisspeptin neuronal populations in the ARC and POA likely play important roles in regulating the GnRH pulse and surge, respectively, in cows.
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DOI：
10.1262/jrd.2012-193
发表时间：
2013
期刊：
The Journal of reproduction and development
影响因子：
--
作者：
Mostari P;Ieda N;Deura C;Minabe S;Yamada S;Uenoyama Y;Maeda K;Tsukamura H
通讯作者：
Tsukamura H
影响因子：
3
作者：
Ohkura, Satoshi;Uenoyama, Yoshihisa;Tsukamura, Hiroko
通讯作者：
Tsukamura, Hiroko
影响因子：
3.6
作者：
Alves, Bruna R. C.;Cardoso, Rodolfo C.;Amstalden, Marcel
通讯作者：
Amstalden, Marcel
DOI：
10.1262/jrd.2013-064
发表时间：
2013-12-17
期刊：
The Journal of reproduction and development
影响因子：
--
作者：
Naniwa Y;Nakatsukasa K;Setsuda S;Oishi S;Fujii N;Matsuda F;Uenoyama Y;Tsukamura H;Maeda K;Ohkura S
通讯作者：
Ohkura S
DOI：
10.1523/jneurosci.0763-09.2009
发表时间：
2009-07-22
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gottsch ML;Navarro VM;Zhao Z;Glidewell-Kenney C;Weiss J;Jameson JL;Clifton DK;Levine JE;Steiner RA
通讯作者：
Steiner RA