Interaction of high‐density lipoprotein with Trypanosoma brucei: Effect of membrane stabilizers
Interaction of high‐density lipoprotein with Trypanosoma brucei: Effect of membrane stabilizers
复制标题
高密度脂蛋白与布氏锥虫的相互作用：膜稳定剂的作用
DOI：
10.1002/jcb.240230107
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发表时间：
1983
影响因子：
4
通讯作者：
M. Rifkin
中科院分区：
文献类型：
--
作者：
M. Rifkin
来自非允许性哺乳动物宿主的血清对血流锥虫的特异性裂解是血清杀锥虫因子（高密度脂蛋白）与锥虫表面相互作用的结果。本文中描述的研究试图进一步定义这种细胞毒性脂蛋白的作用模式。高密度脂蛋白与布氏锥虫的结合在4°C下是瞬时的，并且很容易可逆。结合不是由表面糖蛋白介导的，因为在4°C下去除表面涂层增强了结合，并且没有检测到稳定的糖蛋白-脂蛋白复合物。用交联剂二甲基辛二酰亚胺预处理锥虫使细胞对裂解具有抗性。添加膜稳定药物，如细胞松弛素C、D和E，以及局部麻醉剂（地布卡因、丁卡因和普鲁卡因），也可抑制高密度脂蛋白诱导的细胞溶解。所提供的数据支持以下观点：在37°C下，变异表面糖蛋白（一种完整的膜蛋白）的侧向扩散允许血清敏感细胞中最大的高密度脂蛋白-细胞相互作用，并且由低温或加入细胞松弛素、局部麻醉剂或锌诱导的质膜性质改变抑制了这种相互作用，可能是通过更刚性地锚定的表面糖蛋白分子增加质膜的屏蔽。
The specific lysis of bloodstream trypanosomes by serum from a nonpermissive mammalian host is the result of interaction between the serum trypanocidal factor (high‐density lipoprotein) and the trypanosome surface. The studies described in this paper attempt to define further the mode of action of this cytotoxic lipoprotein. The binding of high‐density lipoprotein to Trypanosoma brucei was instantaneous at 4°C and readily reversible. Binding was not mediated by the surface glycoprotein as removal of the surface coat enhanced binding at 4°C, and no stable glycoprotein‐lipoprotein complex could be detected. Pretreatment of trypanosomes with the cross linker dimethylsuberimidate rendered cells resistant to lysis. Addition of membrane‐stabilizing drugs, such as cytochalasins C, D, and E, and local anesthetics (dibucaine, tetracaine, and procaine), also inhibited high‐density lipoprotein‐induced cell lysis. The data presented support the idea that at 37°C lateral diffusion of the variant surface glycoprotein, an integral membrane protein, allows maximal high‐density lipoprotein‐cell interaction in serum‐sensitive cells, and that altered properties of the plasma membrane induced by low temperature or the addition of cytochalasins, local anesthetics, or zinc inhibit this interaction, possibly by increasing the shielding of the plasma membrane by more rigidly anchored surface glycoprotein molecules.
人类利什曼病的体外寄生虫-单核细胞相互作用。
DOI：
10.1172/jci110606
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Wyler,DJ
通讯作者：
Wyler,DJ
人高密度脂蛋白 (HDL3) 与大鼠肝脏质膜结合。
DOI：
10.1016/0005-2760(82)90094-7
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Chacko,GK
通讯作者：
Chacko,GK