Escherichia coli Heat-Labile Enterotoxin B Subunit Combined with Ginsenoside Rg1 as an Intranasal Adjuvant Triggers Type I Interferon Signaling Pathway and Enhances Adaptive Immune Responses to an Inactivated PRRSV Vaccine in ICR Mice.
Escherichia coli Heat-Labile Enterotoxin B Subunit Combined with Ginsenoside Rg1 as an Intranasal Adjuvant Triggers Type I Interferon Signaling Pathway and Enhances Adaptive Immune Responses to an Inactivated PRRSV Vaccine in ICR Mice.
复制标题
DOI：
10.3390/vaccines9030266
复制
发表时间：
2021-03-16
期刊：
影响因子：
7.8
通讯作者：
Yuan X
中科院分区：
文献类型：
--
作者：
Su F;Wu Y;Li J;Huang Y;Yu B;Xu L;Xue Y;Xiao C;Yuan X
猪繁殖与呼吸综合征病毒（PRRSV）是一种威胁全球养猪业近30年的主要病原体。由于目前的疫苗不能提供完全的保护，因此迫切需要探索新的预防战略。在这里，我们将大肠杆菌（LTB）的热不稳定肠毒素B亚基与人参皂苷Rg1结合形成鼻内佐剂，并评估了将其添加到灭活的prrsv疫苗中对小鼠免疫应答的增强作用。与单独接种PRRSV疫苗相比，联合佐剂协同引起循环系统和呼吸道中更高的中和和非中和（免疫球蛋白G和A）抗体反应，增强脾脏和肺组织中T和B淋巴细胞增殖、CD4+ T细胞启动和细胞毒性CD4+ T细胞活性，并导致Th1/Th2/Th17反应平衡。更重要的是，我们观察到联合佐剂也上调了I型干扰素信号，这可能有助于改善适应性免疫反应。这些结果突出了联合佐剂方法在提高PRRSV疫苗接种效力方面的潜在价值。需要进一步的研究来评估这种联合佐剂在猪身上的效果。
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen that has threatened the global swine industry for almost 30 years. Because current vaccines do not provide complete protection, exploration of new preventive strategies is urgently needed. Here, we combined a heat-labile enterotoxin B subunit of Escherichia coli (LTB) and ginsenoside Rg1 to form an intranasal adjuvant and evaluated its enhancement of immune responses in mice when added to an inactivated-PRRSV vaccine. The combination adjuvant synergistically elicited higher neutralizing and non-neutralizing (immunoglobulin G and A) antibody responses in the circulatory system and respiratory tract, and enhanced T and B lymphocyte proliferation, CD4+ T-cell priming, and cytotoxic CD4+ T cell activities in mononuclear cells from spleen and lung tissues when compared to the PRRSV vaccine alone, and it resulted in balanced Th1/Th2/Th17 responses. More importantly, we observed that the combination adjuvant also up-regulated type I interferon signaling, which may contribute to improvement in adaptive immune responses. These results highlight the potential value of a combined adjuvant approach for improving the efficacy of vaccination against PRRSV. Further study is required to evaluate the efficacy of this combined adjuvant in swine.
登录
查看更多内容
影响因子：
5.4
作者：
Huang, Chen;Zhang, Qiong;Feng, Wen-hai
通讯作者：
Feng, Wen-hai
影响因子：
5.5
作者：
Hagiwar, Y;Tsuji, T;Tamura, S
通讯作者：
Tamura, S
使用活重组乳酸乳球菌结合不耐热毒素 B 亚基进行鼻内免疫可保护鸡免受高致病性禽流感 H5N1 病毒的侵害。
影响因子：
12.7
作者：
Lei, Han;Peng, Xiaojue;Zhao, Daxian
通讯作者：
Zhao, Daxian
影响因子：
5.4
作者：
Johnson, Susan;Eller, Michael;Streeck, Hendrik
通讯作者：
Streeck, Hendrik
影响因子：
3.7
作者：
Lewis DJ;Huo Z;Barnett S;Kromann I;Giemza R;Galiza E;Woodrow M;Thierry-Carstensen B;Andersen P;Novicki D;Del Giudice G;Rappuoli R
通讯作者：
Rappuoli R