6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection
6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection
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6-氮杂-2-硫代-胸腺嘧啶稳定金纳米簇作为蛋白质检测的光致发光探针
DOI：
10.3390/nano10020281
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发表时间：
2020-02
期刊：
影响因子：
5.3
通讯作者：
Chen Wei
中科院分区：
文献类型：
--
作者：
Deng Hao-Hua;Shi Xiao-Qiong;Balasubramanian Paramasivam;Huang Kai-Yuan;Xu Ying-Ying;Huang Zhong-Nan;Peng Hua-Ping;Chen Wei
This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).
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