Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120.
Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120.
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DOI：
10.3390/molecules26102893
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发表时间：
2021-05-13
期刊：
影响因子：
--
通讯作者：
Yang KL
中科院分区：
文献类型：
--
作者：
Abbasi AD;Hussain Z;Yang KL
我们报告了一种无标记和简单的方法，用于检测糖蛋白-120（gp-120）使用适配体为基础的液晶（LC）生物传感平台。将LC与适量的B40t77适体一起支撑在改性载玻片的表面上，允许LC垂直排列。由于B40t77和gp-120之间的特异性相互作用，在表面上观察到明显的拓扑变化，这导致LC的垂面排列的破坏。这导致在偏光显微镜下观察到的暗到亮的转变。利用所开发的生物传感平台，不仅可以识别gp-120，而且可以通过图像分析对所获得的结果进行定量分析。研究表明，拟定生物传感平台的检测限为0.2 µ g/mL gp-120。关于开发平台的选择性，当gp-120被其他蛋白质（如牛血清白蛋白（BSA）、甲型肝炎病毒衣壳蛋白1（Hep A VP1）和免疫球蛋白G蛋白（IgG））替代时，未检测到响应。由于无标记、高特异性和不需要仪器读出等属性，所提出的生物传感平台提供了开发用于快速检测HIV-1 gp-120的工作装置的潜力。
We report a label-free and simple approach for the detection of glycoprotein-120 (gp-120) using an aptamer-based liquid crystals (LCs) biosensing platform. The LCs are supported on the surface of a modified glass slide with a suitable amount of B40t77 aptamer, allowing the LCs to be homeotropically aligned. A pronounced topological change was observed on the surface due to a specific interaction between B40t77 and gp-120, which led to the disruption of the homeotropic alignment of LCs. This results in a dark-to-bright transition observed under a polarized optical microscope. With the developed biosensing platform, it was possible to not only identify gp-120, but obtained results were analyzed quantitatively through image analysis. The detection limit of the proposed biosensing platform was investigated to be 0.2 µg/mL of gp-120. Regarding selectivity of the developed platform, no response could be detected when gp-120 was replaced by other proteins, such as bovine serum albumin (BSA), hepatitis A virus capsid protein 1 (Hep A VP1) and immunoglobulin G protein (IgG). Due to attributes such as label-free, high specificity and no need for instrumental read-out, the presented biosensing platform provides the potential to develop a working device for the quick detection of HIV-1 gp-120.
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影响因子：
56.9
作者：
Feng, Y;Broder, CC;Berger, EA
通讯作者：
Berger, EA
影响因子：
1.5
作者：
Rychert, Jenna;Strick, Daryld;Rosenberg, Eric
通讯作者：
Rosenberg, Eric
DOI：
10.3390/ma11010020
发表时间：
2017-12-23
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Popov N;Honaker LW;Popova M;Usol'tseva N;Mann EK;Jákli A;Popov P
通讯作者：
Popov P
影响因子：
3.9
作者：
Chen, Chih-Hsin;Yang, Kun-Lin
通讯作者：
Yang, Kun-Lin
影响因子：
8.4
作者：
Kim, Hyeong Jin;Jang, Chang-Hyun
通讯作者：
Jang, Chang-Hyun