Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants.
Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants.
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DOI：
10.1038/s41551-022-00889-z
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发表时间：
2022-08
影响因子：
28.1
通讯作者：
Myhrvold, Cameron
中科院分区：
文献类型：
--
作者：
Arizti-Sanz, Jon;Bradley, A'Doriann;Zhang, Yibin B.;Boehm, Chloe K.;Freije, Catherine A.;Grunberg, Michelle E.;Kosoko-Thoroddsen, Tinna-Solveig F.;Welch, Nicole L.;Pillai, Priya P.;Mantena, Sreekar;Kim, Gaeun;Uwanibe, Jessica N.;John, Oluwagboadurami G.;Eromon, Philomena E.;Kocher, Gregory;Gross, Robin;Lee, Justin S.;Hensley, Lisa E.;MacInnis, Bronwyn L.;Johnson, Jeremy;Springer, Michael;Happi, Christian T.;Sabeti, Pardis C.;Myhrvold, Cameron
严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的广泛传播和进化要求快速的核酸诊断方法，这种方法易于在集中式临床实验室之外使用。在这里，我们报告了基于Cas13的核酸分析的开发和性能基准，该方法利用冷冻干燥试剂和常温下快速样品灭活。我们将这些检测方法命名为SHINEv.2(意为“简化的感染高亮显示，以应对流行病，版本2”)，通过消除加热步骤和试剂的冷藏需求，简化了之前报道的不提取RNA的SHINEv.1技术。SHINEv.2在不到90分钟的时间内检测出鼻咽样本中的SARS-CoV-2，灵敏度为90.5%，特异度为100%(以逆转录定量聚合酶链式反应为基准)，采用侧流技术，并在37°C的热块中孵育。SHINEv.2还允许视觉区分Alpha、Beta、Gamma、Delta和Omicron SARS-CoV-2变体，并且可以利用体热运行而不会造成性能损失。准确、易于使用和无需设备的核酸检测可以促进在护理地点和家庭环境中对SARS-CoV-2和其他病原体进行更广泛的测试。
The widespread transmission and evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use outside of centralized clinical laboratories. Here we report the development and performance benchmarking of Cas13-based nucleic acid assays leveraging lyophilised reagents and fast sample inactivation at ambient temperature. The assays, which we named SHINEv.2 (for ‘streamlined highlighting of infections to navigate epidemics, version 2’), simplify the previously reported RNA-extraction-free SHINEv.1 technology by eliminating heating steps and the need for cold storage of the reagents. SHINEv.2 detected SARS-CoV-2 in nasopharyngeal samples with 90.5% sensitivity and 100% specificity (benchmarked against the reverse transcription quantitative polymerase chain reaction) in less than 90 min, using lateral-flow technology and incubation in a heat block at 37 °C. SHINEv.2 also allows for the visual discrimination of the Alpha, Beta, Gamma, Delta and Omicron SARS-CoV-2 variants, and can be run without performance losses by using body heat. Accurate, easy-to-use and equipment-free nucleic acid assays could facilitate wider testing for SARS-CoV-2 and other pathogens in point-of-care and at-home settings.
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CRISPR-Cas12a辅助核酸检测
影响因子：
33.5
作者：
Li SY;Cheng QX;Wang JM;Li XY;Zhang ZL;Gao S;Cao RB;Zhao GP;Wang J
通讯作者：
Wang J
影响因子：
64.8
作者：
Ackerman, Cheri M.;Myhrvold, Cameron;Sabeti, Pardis C.
通讯作者：
Sabeti, Pardis C.
DOI：
10.1016/j.ijid.2021.11.007
发表时间：
2022-01
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Ghosh P;Chowdhury R;Hossain ME;Hossain F;Miah M;Rashid MU;Baker J;Rahman MZ;Rahman M;Ma X;Duthie MS;Wahed AAE;Mondal D
通讯作者：
Mondal D
影响因子：
64.5
作者：
Fozouni P;Son S;Díaz de León Derby M;Knott GJ;Gray CN;D'Ambrosio MV;Zhao C;Switz NA;Kumar GR;Stephens SI;Boehm D;Tsou CL;Shu J;Bhuiya A;Armstrong M;Harris AR;Chen PY;Osterloh JM;Meyer-Franke A;Joehnk B;Walcott K;Sil A;Langelier C;Pollard KS;Crawford ED;Puschnik AS;Phelps M;Kistler A;DeRisi JL;Doudna JA;Fletcher DA;Ott M
通讯作者：
Ott M
影响因子：
56.9
作者：
Chen, Janice S.;Ma, Enbo;Doudna, Jennifer A.
通讯作者：
Doudna, Jennifer A.