RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in Salmonella enterica serovar Typhimurium.
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in Salmonella enterica serovar Typhimurium.
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DOI：
10.1186/1471-2180-9-45
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发表时间：
2009-02-26
期刊：
影响因子：
4.2
通讯作者：
Coombes BK
中科院分区：
文献类型：
--
作者：
Osborne SE;Coombes BK
肠道沙门氏菌在细胞内宿主生态位的生存需要高度协调的毒力效应子的表达，主要由SsrAB双组分调控系统调节。S.缺乏ssrAB基因的肠血清型鼠伤寒突变体在小鼠中是无毒的，这突出了该调节系统在体内的重要性。缺乏编码替代σ因子σE（rpoE）的基因的突变体的细胞内存活也高度减弱，这表明与SsrAB调节系统的潜在联系。在这项研究中，我们证明，RpoE是参与微调的表达的一个子集的SsrB调节的基因发现在沙门氏菌致病岛-2（SPI-2）的遗传位点，编码一个水平获得的III型分泌系统，和未连接的基因整合到这个调节子，需要在宿主动物的毒力。这些数据表明，缺乏ssrB和rpoE的菌株的毒力表型之间的潜在联系，并强调新的转录调控，可能是必要的适当的时间和空间控制的毒力相关的III型分泌系统在宿主感染。
The survival of Salmonella enterica within the intracellular host niche requires highly co-ordinated expression of virulence effectors predominantly regulated by the SsrAB two-component regulatory system. S. enterica serovar Typhimurium mutants lacking the ssrAB genes are avirulent in mice, highlighting the importance of this regulatory system in vivo. Mutants lacking the gene encoding the alternative sigma factor σE (rpoE) are also highly attenuated for intracellular survival, pointing to a potential connection with the SsrAB regulatory system. In this study we demonstrate that RpoE is involved in fine-tuning the expression of a subset of SsrB-regulated genes found in the Salmonella pathogenicity island-2 (SPI-2) genetic locus that encodes a horizontally acquired type III secretion system, and unlinked genes integrated into this regulon that are required for virulence in host animals. These data point to a potential connection between the virulence phenotype of strains lacking ssrB and rpoE, and highlight new transcriptional regulation that might be essential for appropriate temporal and spatial control of the virulence-associated type III secretion system during host infection.
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DOI：
10.1073/pnas.0505401102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Coombes, BK;Wickham, ME;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
3.1
作者：
Brumell, JH;Tang, P;Finlay, BB
通讯作者：
Finlay, BB
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
4.8
作者：
Coombes, BK;Brown, NF;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
3.1
作者：
Carlsson, Katrin E.;Liu, Junta;Francis, Matthew S.
通讯作者：
Francis, Matthew S.