Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage.
Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage.
复制标题
DOI：
10.1038/s41598-021-82772-6
复制
发表时间：
2021-02-08
影响因子：
4.6
通讯作者：
Jones RM
中科院分区：
文献类型：
--
作者：
Small KL;Henthorn NT;Angal-Kalinin D;Chadwick AL;Santina E;Aitkenhead A;Kirkby KJ;Smith RJ;Surman M;Jones J;Farabolini W;Corsini R;Gamba D;Gilardi A;Merchant MJ;Jones RM
本文介绍了在欧洲核子研究中心(CERN)的Clear User设备上用100-200 MeV的超高能电子(VHEE)进行的第一次质粒DNA辐照，以确定VHEE的相对生物有效性(RBE)。在干燥和水环境中测量了DNA损伤产额，以确定 ~ 99%的总DNA断裂是由间接效应引起的，这与其他公布的质子和光子测量结果一致。双链断裂(DSB)产率被用作RBE计算的生物学终点，其值被发现与已建立的放射治疗方式一致。VHEE和传统模式在物理损害方面的相似之处使人们相信VHEE的生物效应也将是相似的--这是临床实施的关键。损伤产额被用作使用GEANT4-DNA对VHEE质粒照射的径迹结构进行模拟的基线。目前的DSB产率模型与实验值吻合较好。人们对闪光放射治疗的兴趣与日俱增，促使了对VHEE照射后DSB产额随剂量率变化的研究。在常规和闪光剂量率照射之间没有观察到明显的差异，表明在这些条件下没有看到闪光效应。
This paper presents the first plasmid DNA irradiations carried out with Very High Energy Electrons (VHEE) over 100–200 MeV at the CLEAR user facility at CERN to determine the Relative Biological Effectiveness (RBE) of VHEE. DNA damage yields were measured in dry and aqueous environments to determine that ~ 99% of total DNA breaks were caused by indirect effects, consistent with other published measurements for protons and photons. Double-Strand Break (DSB) yield was used as the biological endpoint for RBE calculation, with values found to be consistent with established radiotherapy modalities. Similarities in physical damage between VHEE and conventional modalities gives confidence that biological effects of VHEE will also be similar—key for clinical implementation. Damage yields were used as a baseline for track structure simulations of VHEE plasmid irradiation using GEANT4-DNA. Current models for DSB yield have shown reasonable agreement with experimental values. The growing interest in FLASH radiotherapy motivated a study into DSB yield variation with dose rate following VHEE irradiation. No significant variations were observed between conventional and FLASH dose rate irradiations, indicating that no FLASH effect is seen under these conditions.
登录
查看更多内容
影响因子：
3.8
作者：
Incerti, S.;Ivanchenko, A.;Zacharatou, C.
通讯作者：
Zacharatou, C.
新型克隆载体的构建和特性分析 2. 多用途克隆系统
影响因子：
3.5
作者：
BOLIVAR, F;RODRIGUEZ, RL;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
3.8
作者：
Chatzipapas KP;Papadimitroulas P;Obeidat M;McConnell KA;Kirby N;Loudos G;Papanikolaou N;Kagadis GC
通讯作者：
Kagadis GC
影响因子：
3.8
作者：
Incerti, S.;Kyriakou, I.;Brown, J. M. C.
通讯作者：
Brown, J. M. C.
影响因子：
3.8
作者：
Bazalova-Carter, Magdalena;Qu, Bradley;Loo, Billy W., Jr.
通讯作者：
Loo, Billy W., Jr.