A Method for Recording Urethral Pressure Profiles in Female Rats.
A Method for Recording Urethral Pressure Profiles in Female Rats.
复制标题
记录雌性大鼠尿道压力曲线的方法
DOI：
10.1371/journal.pone.0140851
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Du G
中科院分区：
文献类型：
--
作者：
Xu S;Li X;Xu L;Chen B;Tan H;Du G
目的尿压曲线(UPP)和漏点压(LPP)测量以及外括约肌(EUS)、肌电(EMG)和视频尿流动力学分析是评价人体尿路功能的主要方法。然而，在雌性大鼠身上记录UPP是一种广泛使用的动物模型，由于它们的身体较小，因此具有挑战性。本研究报道了一种记录雌性大鼠UPP的新方法。材料与方法17只麻醉雌性大鼠。纳入14只大鼠的LPP数据。另3只大鼠因死亡或泌尿生殖器官异常而被排除。使用改良的水灌流导管系统记录UPP曲线，侧孔以随机顺序朝向3、6、9和12点位置。分析LPP、功能性尿路长度(FUL)和最大尿路闭合压(MUCP)。结果平均LPP为64.39±20.29 cm H2O。3、6、9、12点FUL和MUCP值分别为12.90±1.20、16.70±1.95、13.90±2.42和11.60±0.97 mm，38.70±11.85、33.90±11.82、37.40±11.95和71.90±23.01 cm H2O。6点位置的FUL和12点位置的MUCP显著大于其他3个位置。UPP重复记录的FUL和MUCP与第一次记录的FUL和MUCP无显著差异。结论以水灌流导管系统为基础的改良方法记录雌性大鼠的UPP是可行的，且具有可重复性。它所产生的UPP曲线能灵敏地、略微地反映尿路内不同点的详细压力变化，从而提供了详细评估尿路结构，特别是尿道括约肌的机会。这些结果可能会增强雌性大鼠模型在研究尿括约肌机制方面的实用性。
Aims Urethral pressure profile (UPP) and leak-point pressure (LPP) measurements as well as external urethral sphincter (EUS) electromyography (EMG) and videourodynamic analyses are the primary methods for evaluating urethral function in humans. However, UPP recording in female rats, a widely used animal model, is challenging due to their small body sizes. This study reports a novel method for recording UPP in female rats. Materials and Methods Seventeen anesthetized female rats were studied. LPP data for 14 rats were included. The other 3 rats were excluded because of death or abnormal urogenital organs. UPP curves were recorded using a modified water-perfusion catheter system, with the lateral hole facing the 3-, 6-, 9-, and 12-o’clock positions in a randomized sequence. LPP, functional urethral length (FUL) and maximum urethral closure pressure (MUCP) were analyzed. Results The mean LPP was 64.39 ± 20.29 cm H2O. The mean FUL and MUCP values at the 3-, 6-, 9-, and 12-o’clock positions were 12.90 ± 1.20, 16.70 ± 1.95, 13.90 ± 2.42, and 11.60 ± 0.97 mm, respectively, and 38.70 ± 11.85, 33.90 ± 11.82, 37.40 ± 11.95, and 71.90 ± 23.01 cm H2O, respectively. The FUL at the 6-o’clock position and MUCP at the 12-o’clock position were significantly greater than those at the other 3 positions. The FUL and MUCP of repeated UPP recordings were not significantly different than those of the first recordings. Conclusions UPP recording using a modified method based on a water-perfusion catheter system is feasible and replicable in female rats. It produces UPP curves that sensitively and appreciably reflect detailed pressure changes at different points within the urethra and thus provides opportunity to evaluate urethral structures, especially the urethral sphincter, in detail. These results may enhance the utility of female rat models in research of urinary sphincter mechanisms.
影响因子：
3.3
作者：
Chang HY;Havton LA
通讯作者：
Havton LA