Anatomical tracer injections into the lower urinary tract may compromise cystometry and external urethral sphincter electromyography in female rats.
Anatomical tracer injections into the lower urinary tract may compromise cystometry and external urethral sphincter electromyography in female rats.
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DOI：
10.1016/j.neuroscience.2009.11.037
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发表时间：
2010-03-10
期刊：
影响因子：
3.3
通讯作者：
Havton LA
中科院分区：
文献类型：
--
作者：
Chang HY;Havton LA
生理学和解剖学研究通常在实验模型中结合起来。在研究下尿路 (LUT) 时，通常需要进行尿动力学研究和逆行标记的神经元支配周围目标器官。然而，尚不清楚使用解剖示踪剂来标记例如。脊髓神经元可能会干扰排尿反射生理研究的解释。我们在将 5% 氟金 (FG) 溶液或载体注射到主要盆腔神经节 (MPG) 或 EUS 后 5-7 天，在乌拉坦麻醉下对成年雌性大鼠进行膀胱测压和尿道外括约肌 (EUS) 肌电图 (EMG)。 FG 和车辆注入 MPG 和 EUS 会导致排空效率降低。 MPG 注射增加了膀胱收缩的持续时间和收缩间期。 EUS 注射减少了排尿期间 EUS 肌电图的爆发活动，并增加了膀胱收缩的持续时间和最大膀胱内压力。此外，在 FG 组和媒介物组中注射 MPG 或 EUS 后，膀胱重量和大小均增加。我们的结论是，将解剖示踪剂注射到 MPG 和 EUS 中可能会影响对后续尿动力学研究的解释，并建议研究人员考虑实验设计，允许在将解剖示踪剂注射到 LUT 之前进行生理评估。
Physiological and anatomical investigations are commonly combined in experimental models. When studying the lower urinary tract (LUT), it is often of interest to perform both urodynamic studies and retrogradely labeled neurons innervating the peripheral target organs. However, it is not known whether the use of anatomical tracers for the labeling of e.g. spinal cord neurons may interfere with the interpretation of the physiological studies on micturition reflexes. We performed cystometry and external urethral sphincter (EUS) electromyography (EMG) under urethane anesthesia in adult female rats at 5-7 days after injection of a 5% fluorogold (FG) solution or vehicle into the major pelvic ganglia (MPG) or the EUS. FG and vehicle injections into the MPG and EUS resulted in decreased voiding efficiency. MPG injections increased the duration of both bladder contractions and the inter-contractile intervals. EUS injections decreased EUS EMG bursting activity during voiding as well as increased both the duration of bladder contractions and the maximum intravesical pressure. In addition, the bladder weight and size were increased after either MPG or EUS injections in both the FG and vehicle groups. We conclude that the injection of anatomical tracers into the MPG and EUS may compromise the interpretation of subsequent urodynamic studies and suggest investigators to consider experimental designs, which allow for physiological assessments to precede the administration of anatomical tracers into the LUT.
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影响因子：
5.3
作者：
Jiang, Hai-Hong;Gustilo-Ashby, A. Marcus;Salcedo, Levilester B.;Pan, Hui Q.;Sypert, David F.;Butler, Robert S.;Damaser, Margot S.
通讯作者：
Damaser, Margot S.
DOI：
10.1152/ajpregu.1998.274.2.r389
发表时间：
1998-02-01
影响因子：
2.8
作者：
Persson, K;Alm, P;Andersson, KE
通讯作者：
Andersson, KE
DOI：
10.1152/ajprenal.00176.2007
发表时间：
2007-11-01
影响因子：
4.2
作者：
Damaser, Margot S.;Samplaski, Mary K.;Kerns, James M.
通讯作者：
Kerns, James M.
影响因子：
3.6
作者：
KEAST, JR;BOOTH, AM;DEGROAT, WC
通讯作者：
DEGROAT, WC
影响因子：
2
作者：
Jiang, Hai-Hong;Pan, Hui Q.;Gustilo-Ashby, Marcus A.;Gill, Bradley;Glaab, Jonathan;Zaszczurynski, Paul;Damaser, Margot
通讯作者：
Damaser, Margot