Use of an oral stable isotope label to confirm variation in red blood cell mean age that influences HbA1c interpretation.
Use of an oral stable isotope label to confirm variation in red blood cell mean age that influences HbA1c interpretation.
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DOI：
10.1002/ajh.23866
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发表时间：
2015-01
影响因子：
12.8
通讯作者：
Cohen, Robert M.
中科院分区：
文献类型：
--
作者：
Khera, Paramjit K.;Smith, Eric P.;Lindsell, Christopher J.;Rogge, Mary Colleen;Haggerty, Shannon;Wagner, David A.;Palascak, Mary B.;Mehta, Shilpa;Hibbert, Jacqueline M.;Joiner, Clinton H.;Franco, Robert S.;Cohen, Robert M.
HbA1c is commonly used to monitor glycemic control. However, there is growing evidence that the relationship between HbA1c and mean blood glucose (MBG) is influenced by variation in red blood cell (RBC) lifespan in hematologically normal individuals. Correction of HbA1c for mean RBC age (MRBC) requires a noninvasive, accurate, and affordable method to measure RBC survival. In this study, we evaluated whether a stable isotope approach would satisfy these requirements. RBC lifespan and MRBC were determined in a group of nine hematologically normal diabetic and nondiabetic subjects using oral 15N-glycine to label heme in an age cohort of RBC. The MRBC was 58.7 ± 9.1 (2SD) days and RBC lifespan was 106 ± 21 (2SD) days. This degree of variation (±15 - 20%) is consistent with previous studies using other techniques. In a subset of seven subjects, MRBC determined with the biotin label technique were available from approximately five years prior, and strongly correlated with the stable isotope values (R2 = 0.79). This study suggests that the MRBC is stable over time but varies substantially among individuals, and supports the importance of its variation in HbA1c interpretation. The characteristics of the stable isotope method support its suitability for studies to directly evaluate the impact of variation in MRBC on the interpretation of HbA1c.
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影响因子：
158.5
作者：
Holman, Rury R.;Paul, Sanjoy K.;Neil, H. Andrew W.
通讯作者：
Neil, H. Andrew W.
影响因子：
15.9
作者：
GARLICK, RL;MAZER, JS;BUNN, HF
通讯作者：
BUNN, HF
影响因子：
158.5
作者：
Gerstein, Hertzel C.;Miller, Michael E.;Friedewald, William T.
通讯作者：
Friedewald, William T.
影响因子：
2.9
作者：
Mock, DM;Lankford, GL;Strauss, RG
通讯作者：
Strauss, RG
影响因子：
1.8
作者：
Mohas, M.;Kisfali, P.;Wittmann, I.
通讯作者：
Wittmann, I.