Microevolution of Virulence-Related Genes in Helicobacter pylori Familial Infection.
Microevolution of Virulence-Related Genes in Helicobacter pylori Familial Infection.
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DOI：
10.1371/journal.pone.0127197
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kobayashi I
中科院分区：
文献类型：
--
作者：
Furuta Y;Konno M;Osaki T;Yonezawa H;Ishige T;Imai M;Shiwa Y;Shibata-Hatta M;Kanesaki Y;Yoshikawa H;Kamiya S;Kobayashi I
幽门螺杆菌（Helicobacter pylori）是一种可感染人类胃引起胃炎、溃疡和癌症的细菌病原体，已知由于突变和重组而具有高度的基因组/表观基因组多样性。这种细菌通常在儿童时期感染，并在宿主的一生中持续存在。H.幽门螺杆菌的另一个重要特征是，它为了适应新的宿主而急剧改变其基因组。研究了红腹果蝇的微进化和适应性。pylori基因组中，我们在多位点序列分型（MLST）中对由日本父母和子女组成的同一家庭成员中的7个基因进行了相同或非常相似序列类型的全基因组测序。核苷酸替换的检测揭示了涉及儿童的可能传播途径。结果发现，在与毒力相关的基因（cag基因、vacA、hcpDX、tnfα、ggt、htrA和胶原酶基因）、外膜蛋白（OMP）基因和其他细胞表面相关蛋白基因、信号转导基因和限制性修饰基因中发现了非同义（氨基酸改变）突变。我们重建了H.幽门螺杆菌可以适应一个新的人类宿主，我们的研究结果提出了一种可能性，即毒力相关基因的突变变化在适应儿童宿主中起作用。限制修饰基因的变化可能会重塑甲基化组和转录组，以帮助适应。本研究为H.幽门螺杆菌的传播和毒力，并对基础研究和临床实践具有影响。
Helicobacter pylori, a bacterial pathogen that can infect human stomach causing gastritis, ulcers and cancer, is known to have a high degree of genome/epigenome diversity as the result of mutation and recombination. The bacteria often infect in childhood and persist for the life of the host. One of the reasons of the rapid evolution of H. pylori is that it changes its genome drastically for adaptation to a new host. To investigate microevolution and adaptation of the H. pylori genome, we undertook whole genome sequencing of the same or very similar sequence type in multi-locus sequence typing (MLST) with seven genes in members of the same family consisting of parents and children in Japan. Detection of nucleotide substitutions revealed likely transmission pathways involving children. Nonsynonymous (amino acid changing) mutations were found in virulence-related genes (cag genes, vacA, hcpDX, tnfα, ggt, htrA and the collagenase gene), outer membrane protein (OMP) genes and other cell surface-related protein genes, signal transduction genes and restriction-modification genes. We reconstructed various pathways by which H. pylori can adapt to a new human host, and our results raised the possibility that the mutational changes in virulence-related genes have a role in adaptation to a child host. Changes in restriction-modification genes might remodel the methylome and transcriptome to help adaptation. This study has provided insights into H. pylori transmission and virulence and has implications for basic research as well as clinical practice.
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影响因子：
4.5
作者：
Furuta Y;Namba-Fukuyo H;Shibata TF;Nishiyama T;Shigenobu S;Suzuki Y;Sugano S;Hasebe M;Kobayashi I
通讯作者：
Kobayashi I
影响因子：
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作者：
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影响因子：
7.7
作者：
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通讯作者：
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影响因子：
29.4
作者：
Gong, Min;Ling, Samantha Shi Min;Ho, Bow
通讯作者：
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DOI：
10.1073/pnas.1018444108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Kennemann, Lynn;Didelot, Xavier;Suerbaum, Sebastian
通讯作者：
Suerbaum, Sebastian