Determining tissue conductivity in tissue ablation by nanosecond pulsed electric fields.
Determining tissue conductivity in tissue ablation by nanosecond pulsed electric fields.
复制标题
DOI：
10.1016/j.bioelechem.2021.107949
复制
发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Jiang C
中科院分区：
文献类型：
--
作者：
Oshin EA;Guo S;Jiang C
纳秒脉冲电场 (nsPEF) 会导致细胞膜透化，并已用于非热处理癌组织。据报道，膜通透性的增加伴随着阻抗的变化，因此可以通过组织电导率的变化来监测 nsPEF 对组织的消融作用。在本研究中，通过从受到多个 100 ns、1-10 kV 脉冲的小鼠模型离体测定马铃薯和 4T1-luc 乳腺肿瘤组织的电导率，评估 nsPEF 对生物组织的影响。使用校准电极常数为 1.1±0.1 cm 的四针电极系统，通过治疗前后的网络分析仪测量以及实时电压-电流测量来确定组织的电导率。在 0.1-3 MHz 的频率范围内测量马铃薯组织的电导率，电导率随着脉冲数和电压幅度的增加而增加。还观察到肿瘤组织的电导率在相似的频率范围内随着脉冲数量和脉冲电压的增加而增加。此外，经处理的马铃薯组织中的消融区域与组织中的电导率变化之间的线性相关性表明，对正在处理的生物组织进行电导率分析可能是评估 nsPEF 治疗有效性的快速而灵敏的方法。
Nanosecond pulsed electric field (nsPEF) causes the permeabilization of the cell membrane and has been used to non-thermally treat cancerous tissues. As increased permeabilization in membranes were reported to be accompanied by impedance changes, the ablation effect of nsPEF on tissues can be monitored via the changes in tissue conductivity. In this study, effects of nsPEF on biological tissues were evaluated by determining the conductivities of potato and 4T1-luc breast tumor tissues ex vivo from a murine model subjected to multiple 100-ns, 1–10 kV pulses. Using a four-needle electrode system with a calibrated electrode constant of 1.1±0.1 cm, the conductivities of tissues was determined from both the network-analyzer measurement, before and after treatment, and voltage-current measurement in real-time. The conductivity of the potato tissue was measured for a frequency range of 0.1–3 MHz, and it increased with increasing pulse number and voltage amplitude. The conductivity of the tumor tissue was also observed to increase with pulse number and pulse voltage over a similar frequency range. In addition, the linear correlation between the ablation area in a treated potato tissue and the conductivity change in the tissue suggests that conductivity analysis of biological tissue under treatment could be a fast and sensitive approach to evaluate the effectiveness of a nsPEF treatment.
登录
查看更多内容
影响因子：
4.6
作者：
Edelblute CM;Hornef J;Burcus NI;Norman T;Beebe SJ;Schoenbach K;Heller R;Jiang C;Guo S
通讯作者：
Guo S
影响因子：
2.8
作者：
Garcia, P. A.;Pancotto, T.;Davalos, R. V.
通讯作者：
Davalos, R. V.
影响因子：
4.6
作者：
Rossi, Alessandra;Pakhomova, Olga N.;Muratori, Claudia
通讯作者：
Muratori, Claudia
影响因子：
4.6
作者：
Davalos, RV;Rubinsky, B;Otten, DM
通讯作者：
Otten, DM
DOI：
10.1016/j.jamcollsurg.2013.10.013
发表时间：
2014-02-01
影响因子：
5.2
作者：
Dunki-Jacobs, Erik M.;Philips, Prejesh;Martin, Robert C. G., II
通讯作者：
Martin, Robert C. G., II