Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons.
Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons.
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DOI：
10.1016/j.bbih.2022.100451
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发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Imamura F
中科院分区：
文献类型：
--
作者：
LaFever BJ;Kawasawa YI;Ito A;Imamura F
慢性鼻窦炎等条件下的慢性嗅炎(COI)严重损害嗅觉系统的功能和解剖成分。鼻腔注射脂多糖(LPS)可导致嗅球(OB)萎缩、胶质增生和促炎细胞因子的产生。虽然慢性鼻-鼻窦炎患者的OB较小，但嗅觉炎症对OB神经元的影响在很大程度上是未知的。在这项研究中，我们研究了COI对OB投射神经元、二尖瓣细胞(MC)和簇状细胞(TCS)的神经学影响。为诱导COI，我们对小鼠进行了4周和10周的单侧鼻腔注射内毒素。用RNA测序和免疫组织化学方法检测COI对OB的影响。4周后，我们发现反复注射脂多糖可上调去卵巢小鼠免疫相关的生物学通路。我们还发现，COI后10周，OB的TC侧树突长度明显减少。COI后10周，TCS轴突起始段数量减少，长度减少。然而，MC的外侧树突和轴突起始段基本上没有受到影响。此外，在10周的恢复期后，树突分枝和AIS重建都发生了。我们的发现表明，嗅觉炎症特别影响TCS及其集成电路，而MCs可能受到保护，免受这种疾病的影响。这一数据显示了OBS在应对压力时接受神经可塑性改变的能力的独特特征。早期慢性嗅炎激活了嗅球中由干扰素-γ驱动的炎症通路。丛生细胞经历轴突失调，以应对慢性嗅觉炎症。外丛状层的二尖瓣细胞和中间神经元基本不受慢性嗅炎的影响。丛生细胞在一段时间的炎症停止后，从轴突失调中完全恢复
Chronic olfactory inflammation (COI) in conditions such as chronic rhinosinusitis significantly impairs the functional and anatomical components of the olfactory system. COI induced by intranasal administration of lipopolysaccharide (LPS) results in atrophy, gliosis, and pro-inflammatory cytokine production in the olfactory bulb (OB). Although chronic rhinosinusitis patients have smaller OBs, the consequences of olfactory inflammation on OB neurons are largely unknown. In this study, we investigated the neurological consequences of COI on OB projection neurons, mitral cells (MCs) and tufted cells (TCs). To induce COI, we performed unilateral intranasal administration of LPS to mice for 4 and 10 weeks. Effects of COI on the OB were examined using RNA-sequencing approaches and immunohistochemical analyses. We found that repeated LPS administration upregulated immune-related biological pathways in the OB after 4 weeks. We also determined that the length of TC lateral dendrites in the OB significantly decreased after 10 weeks of COI. The axon initial segment of TCs decreased in number and in length after 10 weeks of COI. The lateral dendrites and axon initial segments of MCs, however, were largely unaffected. In addition, dendritic arborization and AIS reconstruction both took place following a 10-week recovery period. Our findings suggest that olfactory inflammation specifically affects TCs and their integrated circuitry, whereas MCs are potentially protected from this condition. This data demonstrates unique characteristics of the OBs ability to undergo neuroplastic changes in response to stress. Early-stage chronic olfactory inflammation activates the interferon-γ-driven inflammatory pathways in the olfactory bulb. Tufted cells undergo neurite dysregulation in response to chronic olfactory inflammation. Mitral cells and interneurons in the external plexiform layer are largely unaffected by chronic olfactory inflammation. Tufted cells experience complete recovery from neurite dysregulation following a period of ceased inflammation
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.5
作者：
Burton, Shawn D.;Urban, Nathaniel N.
通讯作者：
Urban, Nathaniel N.
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影响因子：
4.6
作者：
Chen, Hongbin;Lin, Wei;Liu, Nan
通讯作者：
Liu, Nan
影响因子：
5.6
作者：
Da Pozzo, Eleonora;Tremolanti, Chiara;Martini, Claudia
通讯作者：
Martini, Claudia
影响因子：
4.2
作者：
Curt, A;Bruehlmeier, M;Dietz, V
通讯作者：
Dietz, V