Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice.
Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice.
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DOI：
10.18632/aging.101358
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发表时间：
2018-01-04
期刊：
影响因子：
--
通讯作者：
Jaimovich E
中科院分区：
文献类型：
--
作者：
Del Campo A;Contreras-Hernández I;Castro-Sepúlveda M;Campos CA;Figueroa R;Tevy MF;Eisner V;Casas M;Jaimovich E
肌肉减少症是随着年龄的增长肌肉质量和力量的退行性损失。虽然线粒体代谢在肌肉功能和许多疾病发展中的作用已被描述，但线粒体拓扑结构和动力学在肌肉衰老过程中的作用尚未完全了解。这项工作显示了小鼠一生中线粒体分布和骨骼肌功能变化的时间线。我们分离了不同年龄小鼠的指屈肌短肌纤维。线粒体的融合样表型，以及垂直于纤维轴的方向变化，在成虫组与幼虫组和老年组相比是明显的。此外，肌浆网与线粒体之间的接触面积明显增加。随着形态学的改变，线粒体Ca2+静息水平在10-14个月时降低，而在老年组显著增加。这与老年组中线粒体与jsr对数量的减少相一致。我们的研究结果支持线粒体中几种年龄依赖性变化的观点，这些变化在中年时在完全的肌肉减少表型之前得到加强。
Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understood. This work shows a time line of changes in both mitochondrial distribution and skeletal muscle function during mice lifespan. We isolated muscle fibers from flexor digitorum brevis of mice of different ages. A fusion-like phenotype of mitochondria, together with a change in orientation perpendicular to the fiber axis was evident in the Adult group compared to Juvenile and Older groups. Moreover, an increase in the contact area between sarcoplasmic reticulum and mitochondria was evident in the same group. Together with the morphological changes, mitochondrial Ca2+ resting levels were reduced at age 10-14 months and significantly increased in the Older group. This was consistent with a reduced number of mitochondria-to-jSR pairs in the Older group compared to the Juvenile. Our results support the idea of several age-dependent changes in mitochondria that are accentuated in midlife prior to a complete sarcopenic phenotype.
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影响因子：
20.1
作者：
Chen Y;Csordás G;Jowdy C;Schneider TG;Csordás N;Wang W;Liu Y;Kohlhaas M;Meiser M;Bergem S;Nerbonne JM;Dorn GW 2nd;Maack C
通讯作者：
Maack C
影响因子：
10.6
作者：
Grigorescu, SE;Petkov, N;Kruizinga, P
通讯作者：
Kruizinga, P
影响因子：
5.3
作者：
Ibebunjo, Chikwendu;Chick, Joel M.;Glassa, David J.
通讯作者：
Glassa, David J.
影响因子：
4.2
作者：
EVANS, WJ;CAMPBELL, WW
通讯作者：
CAMPBELL, WW
影响因子：
3.4
作者：
Kaja, Simon;van de Ven, Rob C. G.;Plomp, Jaap J.
通讯作者：
Plomp, Jaap J.