TiO2 type influences fibronectin adsorption
TiO2 type influences fibronectin adsorption
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TiO2 类型影响纤连蛋白吸附
DOI：
--
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Mário A. Barbosa
中科院分区：
文献类型：
--
作者：
Susana Sousa;Pedro Moradas;Mário A. Barbosa
人纤连蛋白 (FN) 在植入物的生物整合中发挥着关键作用，因为成功取决于 FN 等蛋白质的吸附 [1]。事实上，FN 可以成为生物材料表面和细胞之间的中介。研究了人纤连蛋白 (FN) 在商业纯钛上的吸附，该钛具有在 H2O2 溶液中形成的氧化钛层 (TiO2 cp) 和溅射在 Si 上的 TiO2 (TiO2 sp)。通过润湿性研究、X射线光电子能谱(XPS)以及用125I、125I-FN对FN进行放射性标记来评估吸附等温线和粘附功。结合的 FN 与游离 FN 的交换性也通过放射性标记技术进行了评估。接触角测定表明，FN 对 TiO2 cp 表面的亲和力高于对 TiO2 sp 的亲和力。正如表面自由能值所预期的那样，对于 TiO2 cp 基底（越亲水），FN 的粘附功较高，而对于 TiO2 sp 基底（越疏水），FN 的粘附功较低。通过两种不同的技术评估吸附等温线：FN (125I-FN) 的放射性标记和 XPS。如放射性标记数据所示，TiO2 cp 比 TiO2 sp 表面吸附更多的 FN。正如粘附功和可交换性研究所表明的那样，FN 分子也更牢固地附着在前表面上。使用 125I-FN 的结果还表明，当溶液中 FN 浓度高于 100 μg/mL 时，FN 会以多层形式吸附。
Human fibronectin (FN) plays a key role in the biointegration of implants as the success depends on adsorption of proteins like FN [1]. Indeed FN can be an intermediary between the biomaterial surface and cells.The adsorption of human fibronectin (FN) on commercially pure titanium with a titanium oxide layer formed in a H2O2 solution (TiO2 cp) and TiO2 sputtered on Si (TiO2 sp) was studied. Adsorption isotherms and the work of adhesion were assessed by wettability studies, X-ray photoelectron spectroscopy (XPS), and by radiolabelling of FN with 125I, 125I-FN. Exchangeability of bound FN by free FN, was also evaluated by the radiolabelling technique.Contact angle determinations have shown that FN displays higher affinity for the TiO2 cp surface than for the TiO2 sp. As expected from the surface free energy values, the work of adhesion of FN is higher for the TiO2 cp substrate, the more hydrophilic one, and lower for the TiO2 sp substrate, the more hydrophobic one.The adsorption isotherms were evaluated by two different techniques: radiolabelling of FN (125I-FN) and XPS. TiO2 cp adsorbs more FN than the TiO2 sp surfaces as shown by the radiolabelling data. FN molecules are also more strongly attached to the former surface as indicated by the work of adhesion and by the exchangeability studies. Results using 125I-FN also suggests that FN adsorbs as a multilayer for FN concentrations in solution higher than 100 μg/mL.
影响因子：
2.9
作者：
UGAROVA, TP;ZAMARRON, C;PLOW, EF
通讯作者：
PLOW, EF
通过共价连接的自组装单层修饰钛表面来调节细胞粘附。
DOI：
10.1002/jbm.820241004
发表时间：
1990
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Sukenik,CN;Balachander,N;Culp,LA;Lewandowska,K;Merritt,K
通讯作者：
Merritt,K