Patterned resonance plasmonic microarrays for high-performance SPR imaging.

Patterned resonance plasmonic microarrays for high-performance SPR imaging.
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DOI:
10.1021/ac200190b
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发表时间:
2011-04-15
影响因子:
7.4
通讯作者:
Cheng, Quan
Cheng, Quan
中科院分区:
化学1区
文献类型:
--
作者:
Abbas, Abdennour;Linman, Matthew J.;Cheng, Quan

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我们报道了一种新的光学平台,它基于定义的三维微孔几何结构内的SPR产生和限制,从而产生无背景共振的SPR图像。该阵列在成像分析和亚纳米厚度分辨率方面显示出极高的信噪比(S/N>80)。获得了1度/0.01RIU的角灵敏度,信号背景比(S/B)提高到20,比最好的文献结果提高了一个数量级。该设计对于厚度分辨率为0.24 nm的支持类脂膜阵列的实时探测是有效的,并允许对共振点仅隔一个像素(~7μm)的微流控电路进行成像分析。高图像质量和独特的芯片几何形状为阵列筛选和使用SPRI检测的生物微流控开辟了新的途径。
We report a novel optical platform based on SPR generation and confinement inside a defined 3-dimensional microwell geometry that leads to background resonance-free SPR images. The array shows an exceptionally high signal-to-noise ratio (S/N>80) for imaging analysis and subnanometric thickness resolution. An angular sensitivity of 1 degree/0.01 RIU has been achieved and the signal to background ratio (S/B) improves to 20, one order of magnitude higher than best literature results. The design proves effective for probing supported lipid membrane arrays in real time with a thickness resolution of 0.24 nm and allows for imaging analysis of microfluidic circuits where resonant spots are separated by only one pixel (~ 7 μm). The high image quality and unique chip geometry open up new avenues for array screening and biomicrofluidics using SPRi detection.
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