Argyrophilic ubiquitinated cytoplasmic inclusions of Leu-7-positive glial cells in olivopontocerebellar atrophy (multiple system atrophy)
Argyrophilic ubiquitinated cytoplasmic inclusions of Leu-7-positive glial cells in olivopontocerebellar atrophy (multiple system atrophy)
复制标题
橄榄脑桥小脑萎缩（多系统萎缩）中 Leu-7 阳性胶质细胞的嗜银泛素化胞质包涵体
作者：
S. Kato;H. Nakamura;Asao Hirano;Hidefumi Ito;J. F. Llena;S. Yen
我们报道了18例橄榄桥脑小脑萎缩（OPCA）（多系统萎缩，MSA）患者胶质细胞中的细胞质包涵体。改良Bielschowsky’s和Bodian’s银浸染技术显示出强烈的嗜银性，在脑桥、小脑白质、中脑、延髓和基底节区以及脑白质和脊髓均可见。54例对照病例均无胶质亲银包涵体。免疫组织化学，这些包涵体被抗泛素抗体强烈标记。其中一些与罗森塔尔纤维（RF）蛋白抗体反应。抗leu -7抗体对泛素化包涵体胶质细胞的细胞质进行了免疫染色，但抗gfap抗体未对细胞质进行免疫染色。在超微结构上，胶质包涵体主要由约24 ~ 40 nm的原纤维组成，在纵剖面上，原纤维沿其长度被亲锇颗粒物质包裹。这些原纤维在横切面呈环状。通常，在纤维横切面的明亮腔内可见直径约5nm的中心颗粒。在含有胶质丝的星形胶质细胞中未见颗粒包裹的原纤维。银浸渍试样的电镜检查表明，颗粒包覆的原纤维对银有很强的亲和力。利用间接免疫过氧化物酶技术与泛素和RF蛋白抗体的免疫电镜显示，与两者对应的电子密集反应产物位于胶质包涵体的成分上。我们观察到leu -7阳性胶质细胞，主要是少突胶质细胞，具有亲银泛素化包涵体，这可能对OPCA（MSA）的病理评价具有重要意义。
SummaryWe described cytoplasmic inclusions in glial cells in 18 patients with olivopontocerebellar atrophy (OPCA) (multiple system atrophy, MSA). These glial inclusions showed intense argyrophilia with modified Bielschowsky's and Bodian's silver impregnation techniques, and were observed in the pons, cerebellar white matter, midbrain, medulla oblongata and basal ganglia, as well as cerebral white matter and spinal cord. None of the 54 control cases had glial argyrophilic inclusions. Immunohistochemically, these inclusions were intensely labeled by anti-ubiquitin antibody. Some of them reacted with an antibody to Rosenthal fiber (RF) protein. The cytoplasm of ubiquitinated inclusion-bearing glial cells was immunostained by anti-Leu-7 antibody, but not by anti-GFAP antibody. Ultrastructurally, the glial inclusions were composed primarily of approximately 24- to 40-nm fibrils, which were coated with osmiophilic granular material along their length in longitudinal section. These fibrils appeared as annuli in cross section. Often, a central granule approximately 5 nm in diameter was seen in the lucent lumen of a cross-sectioned fibril. The granule-coated fibrils were not seen in the glial filament-containing astrocytes. Electron microscopic examination of silver-impregnated specimens revealed that the granule-coated fibrils had strong affinity for silver. Immunoelectron microscopy using the indirect immunoperoxidase techniques with antibodies to ubiquitin and RF protein revealed that the electron-dense reaction products respective to both were located on constituents of glial inclusions. Our observation that Leu-7-positive glial cells, mainly oligodendroglial cells, had argyrophilic ubiquitinated inclusions may be of significance for the evaluation of the pathology of OPCA(MSA).
抗阿尔茨海默病神经原纤维缠结的单克隆抗体。
DOI：
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发表时间：
1985
期刊：
The American journal of pathology
影响因子：
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作者：
Dickson,DW;Kress,Y;Crowe,A;Yen,SH
通讯作者：
Yen,SH
影响因子：
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作者：
PAPASOZOMENOS, SC;BINDER, LI
通讯作者：
BINDER, LI