SARS-CoV-2 Receptors and Entry Genes Are Expressed in the Human Olfactory Neuroepithelium and Brain.
SARS-CoV-2 Receptors and Entry Genes Are Expressed in the Human Olfactory Neuroepithelium and Brain.
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DOI：
10.1016/j.isci.2020.101839
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发表时间：
2020-12-18
期刊：
影响因子：
5.8
通讯作者：
Rodriguez I
中科院分区：
文献类型：
--
作者：
Fodoulian L;Tuberosa J;Rossier D;Boillat M;Kan C;Pauli V;Egervari K;Lobrinus JA;Landis BN;Carleton A;Rodriguez I
报告指出，COVID-19和嗅觉丧失之间存在关联，以及嗅球中存在SARS-CoV-2病毒粒子。为了测试嗅觉神经上皮是否可能代表病毒的靶点，我们从人类嗅觉神经上皮中产生了RNA-seq文库，其中我们发现编码病毒受体血管紧张素转换酶-2（ACE 2）和病毒内化增强子TMPRSS 2的基因的大量表达。我们分析了人类嗅觉单细胞RNA-seq数据集，并确定维持嗅觉感觉神经元完整性的支持细胞表达ACE 2和TMPRSS 2。ACE 2蛋白在人和小鼠嗅组织中的支持细胞亚群中高度表达。最后，我们在小鼠和人类的特定脑细胞类型中发现了ACE 2转录本。因此，在与大脑直接连接的神经元感觉系统中，支持细胞代表了SARS-CoV-2的潜在入口。SARS-CoV-2受体ACE 2和TMPRSS 2在嗅觉神经上皮细胞中表达ACE 2和TMPRSS 2在支持支持细胞中共表达脑中神经元和非神经元细胞的子集转录ACE 2生物科学;微生物学;病毒学;细胞生物学;生物信息学;组学;转录组学
Reports indicate an association between COVID-19 and anosmia, as well as the presence of SARS-CoV-2 virions in the olfactory bulb. To test whether the olfactory neuroepithelium may represent a target of the virus, we generated RNA-seq libraries from human olfactory neuroepithelia, in which we found substantial expression of the genes coding for the virus receptor angiotensin-converting enzyme-2 (ACE2) and for the virus internalization enhancer TMPRSS2. We analyzed a human olfactory single-cell RNA-seq dataset and determined that sustentacular cells, which maintain the integrity of olfactory sensory neurons, express ACE2 and TMPRSS2. ACE2 protein was highly expressed in a subset of sustentacular cells in human and mouse olfactory tissues. Finally, we found ACE2 transcripts in specific brain cell types, both in mice and humans. Sustentacular cells thus represent a potential entry door for SARS-CoV-2 in a neuronal sensory system that is in direct connection with the brain. SARS-CoV-2 receptors ACE2 and TMPRSS2 are expressed in olfactory neuroepithelia ACE2 and TMPRSS2 are coexpressed in supporting sustentacular cells A subset of neuronal and non-neuronal cells in the brain transcribe ACE2 Biological Sciences; Microbiology; Virology; Cell Biology; Bioinformatics; Omics; Transcriptomics
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影响因子：
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影响因子：
15.3
作者：
Gu, Jiang;Gong, Encong;Zhang, Bo;Zheng, Jie;Gao, Zifen;Zhong, Yanfeng;Zou, Wanzhong;Zhan, Jun;Wang, Shenglan;Xie, Zhigang;Zhuang, Hui;Wu, Bingquan;Zhong, Haohao;Shao, Hongquan;Fang, Weigang;Gao, Dongshia;Pei, Fei;Li, Xingwang;He, Zhongpin;Xu, Danzhen;Shi, Xeying;Anderson, Virginia M;Leong, Anthony S-Y
通讯作者：
Leong, Anthony S-Y