The elastic modulus of Matrigel as determined by atomic force microscopy.
The elastic modulus of Matrigel as determined by atomic force microscopy.
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DOI：
10.1016/j.jsb.2009.05.005
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发表时间：
2009-09
影响因子：
3
通讯作者：
Murphy, Christopher J.
中科院分区：
文献类型：
--
作者：
Soofi, Shauheen S.;Last, Julie A.;Liliensiek, Sara J.;Nealey, Paul F.;Murphy, Christopher J.
最近的研究表明，细胞微环境的生物物理特性强烈影响各种基本的细胞行为。细胞外基质（ECM）对机械力的响应，在数学上被描述为弹性模量，被认为在调节和病理细胞行为中起着特别关键的作用。基底膜（BM）是ECM的特化，其充当许多细胞类型（例如所有上皮细胞）的直接界面，并且细胞通过其连接到下面的基质。基质胶是一种市售的BM样复合物，并作为一种容易获得的实验模拟天然BM。然而，在生理条件下Matrigel的局部弹性模量尚未被定义。在这里，我们提出的程序和结果的压痕测试与原子力显微镜（AFM）在水性，温度控制的环境中进行的Matrigel。发现平均模量值约为450 Pa。然而，这一结果比科学文献中报道的宏观剪切储能模量高得多。这种差异的原因被认为是由于测试方法的差异和Matrigel在低于37° C的温度下软化的趋势。
Recent studies indicate that the biophysical properties of the cellular microenvironment strongly influence a variety of fundamental cell behaviors. The extracellular matrix’s (ECM) response to mechanical force, described mathematically as the elastic modulus, is believed to play a particularly critical role in regulatory and pathological cell behaviors. The basement membrane (BM) is a specialization of the ECM that serves as the immediate interface for many cell types (e.g. all epithelial cells) and through which cells are connected to the underlying stroma. Matrigel is a commercially available BM-like complex and serves as an easily accessible experimental simulant of native BMs. However, the local elastic modulus of Matrigel has not been defined under physiological conditions. Here we present the procedures and results of indentation tests performed on Matrigel with atomic force microscopy (AFM) in an aqueous, temperature controlled environment. The average modulus value was found to be approximately 450 Pa.. However, this result is considerably higher than macroscopic shear storage moduli reported in the scientific literature. The reason for this discrepancy is believed to result from differences in test methods and the tendency of Matrigel to soften at temperatures below 37° C.
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影响因子：
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