Association of circulating exosomal miR-122 levels with BAT activity in healthy humans
Association of circulating exosomal miR-122 levels with BAT activity in healthy humans
复制标题
健康人循环外泌体 miR-122 水平与 BAT 活性的关联
DOI：
10.1038/s41598-019-49754-1
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Saito Masayuki
中科院分区：
文献类型：
--
作者：
Okamatsu-Ogura Yuko;Matsushita Mami;Bariuan Jussiaea Valente;Nagaya Kazuki;Tsubota Ayumi;Saito Masayuki
棕色脂肪组织(BAT)在体内脂肪堆积和能量消耗调节中起着重要作用。由于miRNAs在肥胖和相关代谢性疾病发病机制中的作用存在争议，我们分析了健康受试者血清中外体miRNAs，特别参考了BAT活性和体脂水平。受试者为男性志愿者，年龄20~30岁。冷暴露2 h后，用氟脱氧葡萄糖正电子发射断层扫描和计算机断层扫描评估其BAT活性，并以最大标准摄取值(SUVmax)表示。用基因芯片和实时定量聚合酶链式反应分析外体miRNA水平。高BAT活动组(SUV ≧ 3)的miR-12 2-5p水平比低BAT活动组(SUVmax和3)低53%。Pearson相关分析显示，血清miR-122-5p水平与BAT活性和血清高密度脂蛋白胆固醇呈负相关，与年龄、BMI、体脂质量、总胆固醇和甘油三酯水平呈正相关。多元回归分析显示，BAT活性与血清miR-122-5p水平呈正相关，与其他参数无关。这些结果表明，血清外体miR-122-5p水平与BAT活性呈负相关，与肥胖无关。
Brown adipose tissue (BAT) plays an important role in body fat accumulation and the regulation of energy expenditure. Since the role of miRNAs in the pathogenesis of obesity and related metabolic diseases is contentious, we analyzed exosomal miRNAs in serum of healthy subjects with special references to BAT activity and body fat level. Forty male volunteers aged 20–30 years were recruited. Their BAT activity was assessed by fluorodeoxyglucose positron emission tomography and computed tomography after 2 h of cold exposure and expressed as a maximal standardized uptake value (SUVmax). Exosomal miRNA levels was analyzed using microarray and real-time PCR analyses. The miR-122-5p level in the high BAT activity group (SUV ≧ 3) was 53% lower than in the low BAT activity group (SUVmax <3). Pearson’s correlation analysis revealed that the serum miR-122-5p level correlated negatively with BAT activity and the serum HDL-cholesterol, and it correlated positively with age, BMI, body fat mass, and total cholesterol and triglyceride serum levels. Multivariate regression analysis revealed that BAT activity was associated with the serum miR-122-5p level independently of the other parameters. These results reveal the serum exosomal miR-122-5p level is negatively associated with BAT activity independently of obesity.
影响因子：
18.2
作者：
Kajimura S;Saito M
通讯作者：
Saito M
影响因子：
15.9
作者：
Mori, Marcelo A.;Thomou, Thomas;Kahn, C. Ronald
通讯作者：
Kahn, C. Ronald