The serological evidence in humans supports a negligible risk of zoonotic infection from porcine circovirus type 2.
The serological evidence in humans supports a negligible risk of zoonotic infection from porcine circovirus type 2.
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DOI：
10.1016/j.biologicals.2013.09.005
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发表时间：
2013-11
期刊：
影响因子：
1.7
通讯作者：
Zhang, Yanjin
中科院分区：
文献类型：
--
作者：
Burbelo, Peter D.;Ragheb, Jack A.;Kapoor, Amit;Zhang, Yanjin
猪圆环病毒（PCV）有两种基因型，PCV-1和PCV-2。在猪中，PCV-1感染无症状，但PCV-2感染可引起严重的呼吸道疾病和其他病理学。尽管人类摄入PCV污染的食物并通过其他来源暴露于PCV，但PCV-2作为人类和其他物种的人畜共患病因子的潜力尚未得到充分研究。在此，使用荧光素酶免疫沉淀系统（LIPS）检测PCV-2感染的血清学分析，将来自PCV-2衣壳基因的四种重组蛋白作为抗原进行检查。PCV-2-CAP-Δ1是LIPS检测的最佳重组蛋白，其敏感性为93%，特异性为100%。对健康人献血者、马和牛血清样本的检测未能证明存在抗PCV-2抗体。此外，对两个高风险人群（服用猪源性口服补充剂的囊性纤维化患者和接受猪胰岛细胞移植的I型糖尿病患者）的分析显示，没有抗PCV-2抗体的证据。这些结果扩展了LIPS作为鉴定体液应答的稳健方法的广泛使用，并提供了PCV-2在人类中不具有传染性的证据。
There are two porcine circovirus (PCV) genotypes, PCV-1 and PCV-2. In pigs, PCV-1 infection is asymptomatic but PCV-2 infection can cause severe respiratory disease and other pathology. Although humans ingest PCV-contaminated foods and are exposed to PCV through other sources, the potential of PCV-2 as a zoonotic agent in humans and other species has not been fully explored. Here, four recombinant proteins derived from the PCV-2 capsid gene were examined as antigens using the Luciferase Immunoprecipitation System (LIPS) assay for serological analysis of PCV-2 infection. PCV-2-CAP-Δ1 was the optimum recombinant protein in the LIPS assay with a sensitivity of 93% and specificity of 100% using porcine samples. Testing of healthy human blood donors, equine and bovine serum samples failed to demonstrate the presence of anti-PCV-2 antibodies. Additionally, analysis of two high-risk human groups, cystic fibrosis patients taking porcine derived oral supplements and type I diabetes patients who had undergone porcine islet cell transplantation, showed no evidence of anti-PCV-2 antibodies. These results extend the extensively demonstrated use of LIPS as a robust approach for identifying humoral responses and provide evidence that PCV-2 is not infectious in humans.
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影响因子：
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作者：
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