An assessment of the masked message hypothesis: sea urchin egg messenger ribonucleoprotein complexes are efficient templates for in vitro protein synthesis.
An assessment of the masked message hypothesis: sea urchin egg messenger ribonucleoprotein complexes are efficient templates for in vitro protein synthesis.
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对屏蔽信息假说的评估：海胆卵信使核糖核蛋白复合物是体外蛋白质合成的有效模板。
DOI：
10.1016/0012-1606(82)90126-9
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发表时间：
1982
影响因子：
2.7
通讯作者：
M. B. Hille
中科院分区：
文献类型：
--
作者：
R. Moon;M. Danilchik;M. B. Hille
在未受精的海胆卵中，蛋白质合成的速率非常低，尽管蛋白质合成的所有成分都存在。为了确定卵信使RNA是否由于酚溶性抑制剂的“掩蔽”而无法翻译，在小麦胚芽无细胞体系中体外培养了来自红球海胆卵的粗品和纯化的非多聚核糖体信使核糖核蛋白复合物（mRNP）。粗制和纯化的卵mRNP几乎与从mRNP提取的mRNA一样可翻译，表明mRNP没有被掩蔽。通过在不同离子强度的缓冲液中或在蛋白酶和核糖核酸酶抑制剂的存在下分离mRNP，揭示了mRNP及其组分mRNA的相对翻译活性没有差异。此外，体外翻译动力学分析和在不同浓度的K+，Mg ~（2+）和模板下mRNP和mRNA的翻译都表明mRNP是指导蛋白质合成的有效模板。在聚丙烯酰胺凝胶上对体外和体内翻译产物的分离表明，mRNP和从mRNP中提取的mRNA都在体外合成了高分子量多肽，其中一些也在体内合成。虽然海胆卵mRNP可能不会被掩盖，但有几种替代机制可以调节卵中的翻译。
The rate of protein synthesis in unfertilized sea urchin eggs is very low, although all components for protein synthesis are present. To determine whether egg messenger RNAs are unavailable for translation because of “masking” by phenol-soluble inhibitors, crude and purified nonpolyribosomal messenger ribonucleoprotein complexes (mRNPs) from eggs ofStrongylocentrotus purpuratuswere translatedin vitroin a wheat germ cell-free system. Crude and purified egg mRNPs were nearly as translatable as the mRNAs extracted from the mRNPs, suggesting that the mRNPs were not masked. No difference in the relative translational activities of mRNPs and their constituent mRNAs was revealed by isolating the mRNPs in buffers of different ionic strength or in the presence of protease and ribonuclease inhibitors. Furthermore, kinetic analysis of thein vitrotranslation and translation of the mRNPs and mRNAs at several concentrations of K+, Mg2+, and template all indicate that mRNPs are efficient templates for directing protein synthesis. Separation on polyacrylamide gels of the products ofin vitroandin vivotranslation demonstrated that both mRNPs and mRNAs extracted from the mRNPs synthesizedin vitrohigh-molecular-weight polypeptides, some of which were also synthesizedin vivo. Although sea urchin egg mRNPs may not be masked, there are several alternative mechanisms for regulating translation in the egg.
蛋白质合成作为海胆卵受精的早期反应：模型。
影响因子：
2.7
作者：
Raff,RA;Brandis,JW;Huffman,CJ;Koch,AL;Leister,DE
通讯作者：
Leister,DE
Manduca sexta 细胞承诺中的信使 RNA。
影响因子：
2.7
作者：
Chen,AC;Riddiford,LM
通讯作者：
Riddiford,LM
海胆卵和胚胎的翻译控制：囊胚阶段胚胎的起始是速率限制的。
影响因子：
2.7
作者：
Hille,MB;Hall,DC;Yablonka-Reuveni,Z;Danilchik,MV;Moon,RT
通讯作者：
Moon,RT