Fundamental Characteristics of Single-Cell Aging in Diploid Yeast.
Fundamental Characteristics of Single-Cell Aging in Diploid Yeast.
复制标题
DOI：
10.1016/j.isci.2018.08.011
复制
发表时间：
2018-09-28
期刊：
影响因子：
5.8
通讯作者：
Acar M
中科院分区：
文献类型：
--
作者：
Sarnoski EA;Song R;Ertekin E;Koonce N;Acar M
单细胞水平的实验可以阐明在人群水平研究中被掩盖的关于细胞衰老的关键生物学见解。然而，跟踪单个细胞的大量时间要求在历史上阻碍了它们的长期纵向观察。使用微流体装置，自动显微镜监测二倍体酿酒酵母细胞在其整个复制寿命，在这里，我们报告了二倍体酵母单细胞老化的基本特征。我们发现，具有短半衰期与长半衰期的蛋白质随着细胞年龄的增长表现出不同的动力学，并且细胞间基因表达噪音在衰老过程中增加，而细胞内噪音保持不变。一个随机模型提供了定量机制的见解所观察到的噪声动力学和揭示二倍体和单倍体酵母之间的年龄依赖性细胞内噪声的差异。我们的工作阐明了一组典型的表型如何动态变化，而宿主细胞在真实的时间老化，提供了重要的见解，全面了解和控制寿命在单细胞水平。微流控装置有助于对老化二倍体酵母的纵向观察随着细胞老化半衰期短与长的蛋白质表现出不同的动力学在复制老化期间细胞间基因表达噪音增加与单倍体酵母不同，在二倍体酵母老化期间细胞内噪音不变基因网络;系统生物学;生物科学仪器
Single-cell-level experimentation can elucidate key biological insights about cellular aging that are masked in population-level studies. However, the extensive time requirement of tracking single cells has historically prevented their long-term longitudinal observation. Using a microfluidic device that automates microscopic monitoring of diploid Saccharomyces cerevisiae cells throughout their replicative lifespan, here we report the fundamental characteristics of single-cell aging for diploid yeast. We find that proteins with short versus long half-lives exhibit distinct dynamics as cells age and that the intercellular gene expression noise increases during aging, whereas the intracellular noise stays unchanged. A stochastic model provides quantitative mechanistic insights into the observed noise dynamics and sheds light on the age-dependent intracellular noise differences between diploid and haploid yeast. Our work elucidates how a set of canonical phenotypes dynamically change while the host cells are aging in real time, providing essential insights for a comprehensive understanding on and control of lifespan at the single-cell level. A microfluidic device facilitates longitudinal observation of aging diploid yeast Proteins with short versus long half-lives exhibit distinct dynamics as cells age Intercellular gene expression noise increases during replicative aging Unlike haploid yeast, intracellular noise is unchanged during aging in diploid yeast Gene Network; Systems Biology; Biological Science Instrumentation
登录
查看更多内容
影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
DOI：
10.1016/j.cub.2010.04.045
发表时间：
2010-06-22
期刊：
Current biology : CB
影响因子：
--
作者：
Wang P;Robert L;Pelletier J;Dang WL;Taddei F;Wright A;Jun S
通讯作者：
Jun S
影响因子：
23.9
作者：
Siudeja, Katarzyna;Nassari, Sonya;Gervais, Louis;Skorski, Patricia;Lameiras, Sonia;Stolfa, Donato;Zande, Maria;Bernard, Virginie;Frio, Thomas Rio;Bardin, Allison J.
通讯作者：
Bardin, Allison J.
影响因子：
5.3
作者：
SALAMA, SR;HENDRICKS, KB;THORNER, J
通讯作者：
THORNER, J
DOI：
10.1073/pnas.162041399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Swain, PS;Elowitz, MB;Siggia, ED
通讯作者：
Siggia, ED