Optical methods for single molecule detection and analysis.

Optical methods for single molecule detection and analysis.
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DOI:
10.1021/ac3027178
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发表时间:
2013-02-05
影响因子:
7.4
通讯作者:
Walt, David R.
Walt, David R.
中科院分区:
化学1区
文献类型:
--
作者:
Walt, David R.

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作为分析化学家,人们可以进行的最高分辨率测量是在单分子水平上;它只是没有得到任何比这更好的。要确定溶液中分子的浓度,最好的方法是计算给定体积中的分子数量。只要体积包含统计上足够大数量的分子并且高于泊松噪声极限,分子计数就是进行测量的最准确的方法。分子计数是未来的方法,今天开始进行。
As analytical chemists, the highest resolution measurement one can make is at the single molecule level; it just does not get any better than that. To determine the concentration of a molecule in solution, the best way is to count the number of molecules in a given volume. As long as the volume contains a statistically large enough number of molecules and is above the Poisson noise limit, molecular counting is the most accurate way to make a measurement. Molecular counting is the method of the future and is beginning to be performed today.
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