Optical methods for single molecule detection and analysis.
Optical methods for single molecule detection and analysis.
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DOI:
10.1021/ac3027178
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发表时间:
2013-02-05
影响因子:
7.4
通讯作者:
Walt, David R.
中科院分区:
文献类型:
--
作者:
Walt, David R.
As analytical chemists, the highest resolution measurement one can make is at the single molecule level; it just does not get any better than that. To determine the concentration of a molecule in solution, the best way is to count the number of molecules in a given volume. As long as the volume contains a statistically large enough number of molecules and is above the Poisson noise limit, molecular counting is the most accurate way to make a measurement. Molecular counting is the method of the future and is beginning to be performed today.
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