DNA-based diet analysis for any predator.
DNA-based diet analysis for any predator.
复制标题
DOI：
10.1371/journal.pone.0005252
复制
发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Dunshea G
中科院分区：
文献类型：
--
作者：
Dunshea G
食物样本中的猎物DNA可以作为一种饮食标记，但目前的方法猎物检测需要先验的饮食知识和/或设计专案，限制了他们的范围。我提出了一个一般的方法来检测不同的猎物在粪便或肠道内容物的捕食者。在所概述的例子中，我利用了核酸内切酶Pac I的限制性位点，它存在于大多数齿鲸类哺乳动物的16 S mtDNA中，但在大多数其他相关的非哺乳动物脊索动物和无脊椎动物中不存在。因此，在从这些哺乳动物捕食者的粪便中提取的DNA中，Pac I将切割并从由新型通用引物靶向的小区域中排除捕食者DNA，而大多数猎物DNA保持完整，从而允许猎物选择性PCR。该方法进行了优化，使用粪便样品圈养的短吻海豚（Tursiops truncatus）喂养的饮食6-10猎物物种从三个phlya。多达五个猎物从两个门被检测到在一个单一的粪便和所有，但一个小的猎物项目（2%的整体饮食）在所有样品中检测到。同样的方法适用于粪便样品从自由放养的海豚，多达7个猎物类群被发现在一个单一的粪便和13个猎物类群从8硬骨鱼科被确定。提供的数据和进一步的例子，以方便快速转移这种方法的任何捕食者。这种方法应该证明有用的动物学家使用DNA为基础的饮食技术在各种各样的研究系统。
Prey DNA from diet samples can be used as a dietary marker; yet current methods for prey detection require a priori diet knowledge and/or are designed ad hoc, limiting their scope. I present a general approach to detect diverse prey in the feces or gut contents of predators. In the example outlined, I take advantage of the restriction site for the endonuclease Pac I which is present in 16S mtDNA of most Odontoceti mammals, but absent from most other relevant non-mammalian chordates and invertebrates. Thus in DNA extracted from feces of these mammalian predators Pac I will cleave and exclude predator DNA from a small region targeted by novel universal primers, while most prey DNA remain intact allowing prey selective PCR. The method was optimized using scat samples from captive bottlenose dolphins (Tursiops truncatus) fed a diet of 6–10 prey species from three phlya. Up to five prey from two phyla were detected in a single scat and all but one minor prey item (2% of the overall diet) were detected across all samples. The same method was applied to scat samples from free-ranging bottlenose dolphins; up to seven prey taxa were detected in a single scat and 13 prey taxa from eight teleost families were identified in total. Data and further examples are provided to facilitate rapid transfer of this approach to any predator. This methodology should prove useful to zoologists using DNA-based diet techniques in a wide variety of study systems.
登录
查看更多内容
影响因子：
4.9
作者：
Blankenship, LE;Yayanos, AA
通讯作者：
Yayanos, AA
影响因子：
4.4
作者：
Huber, JA;Butterfield, DA;Baross, JA
通讯作者：
Baross, JA
影响因子：
1.7
作者：
Barros, NB;Wells, RS
通讯作者：
Wells, RS
影响因子：
3.7
作者：
Deagle, Bruce E.;Gales, Nick J.;Evans, Karen;Jarman, Simon N.;Robinson, Sarah;Trebilco, Rowan;Hindell, Mark A.
通讯作者：
Hindell, Mark A.
影响因子：
6.1
作者：
Iverson, SJ;Field, C;Blanchard, W
通讯作者：
Blanchard, W