Studying seabird diet through genetic analysis of faeces: a case study on macaroni penguins (Eudyptes chrysolophus).
Studying seabird diet through genetic analysis of faeces: a case study on macaroni penguins (Eudyptes chrysolophus).
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DOI：
10.1371/journal.pone.0000831
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发表时间：
2007-09-05
期刊：
影响因子：
3.7
通讯作者：
Hindell, Mark A.
中科院分区：
文献类型：
--
作者：
Deagle, Bruce E.;Gales, Nick J.;Evans, Karen;Jarman, Simon N.;Robinson, Sarah;Trebilco, Rowan;Hindell, Mark A.
海鸟饮食的测定通常依赖于通过胃冲洗获得的胃内容物残留物的分析;这种技术既有侵入性又有后勤困难。我们评估的有用性的DNA为基础的粪便分析在饮食研究鸡饲养通心粉企鹅（Eudyptes chrysolophus）在赫德岛。还收集了传统的胃内容物数据，以便对这些方法进行比较。利用猎物特异性PCR检测粪便中的食物DNA，并对扩增的猎物DNA进行克隆和测序。在收集的88个粪便样本中，39个样本含有可检测的DNA，这些DNA来自一个或多个被PCR检测的猎物群体。磷虾类DNA是最常见的检测在早期（警卫）阶段的鸡饲养，和DNA的myctophid鱼Krefftichthys andersoni和端足类动物的检测变得更常见的样品中收集的后期（crèche）阶段。这些趋势与在企鹅胃内容物中观察到的趋势一致。在磷虾特异性克隆文库中，两种主要磷虾猎物物种（瓦氏磷虾和长尾鲷）的序列比例在采样期间发生了变化;这再次反映了胃内容物数据的趋势。通用克隆库中的猎物序列分析显示，鱼的猎物比确定在胃中的多样性更高，但非鱼的猎物没有很好的代表。目前的研究是第一次使用基于DNA的粪便分析来检查捕食者饮食的全方位。我们讨论方法上遇到的困难，并提出可能的改进。总的来说，基于DNA的方法检测通心粉企鹅饮食的时间变化的能力表明，这种非侵入性的方法将普遍适用于监测海鸟的人口水平的饮食趋势。
Determination of seabird diet usually relies on the analysis of stomach-content remains obtained through stomach flushing; this technique is both invasive and logistically difficult. We evaluate the usefulness of DNA-based faecal analysis in a dietary study on chick-rearing macaroni penguins (Eudyptes chrysolophus) at Heard Island. Conventional stomach-content data was also collected, allowing comparison of the approaches. Prey-specific PCR tests were used to detect dietary DNA in faecal samples and amplified prey DNA was cloned and sequenced. Of the 88 faecal samples collected, 39 contained detectable DNA from one or more of the prey groups targeted with PCR tests. Euphausiid DNA was most commonly detected in the early (guard) stage of chick-rearing, and detection of DNA from the myctophid fish Krefftichthys anderssoni and amphipods became more common in samples collected in the later (crèche) stage. These trends followed those observed in the penguins' stomach contents. In euphausiid-specific clone libraries the proportion of sequences from the two dominant euphausiid prey species (Euphausia vallentini and Thysanoessa macrura) changed over the sampling period; again, this reflected the trend in the stomach content data. Analysis of prey sequences in universal clone libraries revealed a higher diversity of fish prey than identified in the stomachs, but non-fish prey were not well represented. The present study is one of the first to examine the full breadth of a predator's diet using DNA-based faecal analysis. We discuss methodological difficulties encountered and suggest possible refinements. Overall, the ability of the DNA-based approach to detect temporal variation in the diet of macaroni penguins indicates this non-invasive method will be generally useful for monitoring population-level dietary trends in seabirds.
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影响因子：
4.9
作者：
Blankenship, LE;Yayanos, AA
通讯作者：
Yayanos, AA
影响因子：
2.7
作者：
Cherel, Y;Hobson, KA;Weimerskirch, H
通讯作者：
Weimerskirch, H
影响因子：
1.7
作者：
Ainley, DG;Wilson, PR;Karl, B
通讯作者：
Karl, B
DOI：
10.1098/rsbl.2003.0153
发表时间：
2004-05-07
影响因子：
4.7
作者：
Brooke, MD
通讯作者：
Brooke, MD
影响因子：
2.5
作者：
Clarke, Judy;Emmerson, Louise M.;Otahal, Petr
通讯作者：
Otahal, Petr