Membrane sorting during phagocytosis: selective exclusion of major histocompatibility complex molecules but not complement receptor CR3 during conventional and coiling phagocytosis.
Membrane sorting during phagocytosis: selective exclusion of major histocompatibility complex molecules but not complement receptor CR3 during conventional and coiling phagocytosis.
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吞噬作用期间的膜分选：选择性排除主要的组织相容性复合物分子，但在常规和盘绕吞噬作用期间不补充受体CR3。
DOI：
10.1084/jem.175.5.1317
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发表时间：
1992-05-01
影响因子：
15.3
通讯作者：
HORWITZ, MA
中科院分区：
文献类型：
--
作者：
CLEMENS, DL;HORWITZ, MA
我们利用免疫细胞化学和酶细胞化学技术研究了嗜肺军团菌盘绕吞噬和大肠杆菌常规吞噬过程中质膜蛋白的分布。在常规吞噬和盘式吞噬过程中，新生吞噬体相对排斥I类和II类主要组织相容性复合体（MHC）分子，而CR3补体受体则存在于新生吞噬体中。碱性磷酸酶活性的染色模式与MHC分子相似，在嗜肺乳杆菌盘状和新生吞噬体中明显排除了磷酸酶活性。另一方面，5'-核苷酸酶活性的染色模式与CR3相似，在嗜肺乳杆菌线圈的内层有强烈的染色。这些结果表明，在吞噬过程中，细胞有能力选择性地排除新生吞噬体中的某些膜蛋白，从而产生明显不同于其来源的质膜的吞噬体膜。
We have used immunocytochemical techniques and enzyme cytochemistry to examine the distribution of plasma membrane proteins during coiling phagocytosis of Legionella pneumophila and conventional phagocytosis of Escherichia coli. Whereas class I and class II major histocompatibility complex (MHC) molecules are relatively excluded from nascent phagosomes during conventional and coiling phagocytosis, the CR3 complement receptor persists in nascent phagosomes. The staining pattern for alkaline phosphatase activity resembles that of MHC molecules, with a marked exclusion of phosphatase activity from L. pneumophila coils and nascent phagosomes. The staining pattern for 5'-nucleotidase activity, on the other hand, resembles that of CR3 with intense staining in the inner layers of L. pneumophila coils. These results demonstrate that the cell has the ability to exclude selectively certain membrane proteins from the nascent phagosome during phagocytosis, thereby producing a phagosomal membrane markedly different from the plasma membrane from which it is derived.
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DOI：
10.1084/jem.158.6.2108
发表时间：
1983-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Horwitz MA
通讯作者：
Horwitz MA
影响因子：
2.1
作者：
CHANG, KP
通讯作者：
CHANG, KP
影响因子：
64.5
作者：
HORWITZ, MA
通讯作者：
HORWITZ, MA
影响因子：
4.1
作者：
STOCHAJ, U;FLOCKE, K;MANNHERZ, HG
通讯作者：
MANNHERZ, HG
影响因子：
3.1
作者：
ANTOINE, JC;JOUANNE, C;FREHEL, C
通讯作者：
FREHEL, C