Potential of hydrogen production from sugarcane juice by Ethanoligenens harbinense Yuan-3
Potential of hydrogen production from sugarcane juice by Ethanoligenens harbinense Yuan-3
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哈尔滨产乙醇元3号甘蔗汁产氢潜力
DOI：
10.1016/j.jclepro.2019.07.027
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发表时间：
2019-11
影响因子：
11.1
通讯作者：
Nanqi Ren
中科院分区：
文献类型：
--
作者：
Weiming Li;Chi Cheng;Cao Guangli;Shang-Tian Yang;Nanqi Ren
摘要廉价的生物质是低成本生产生物氢的理想底物，但乙醇型产氢细菌Ethanoligenens harbinense的生物质利用性能却知之甚少。研究了利用甘蔗汁发酵产氢的可行性和产氢性能。首次以SJ为碳源，对发酵培养基和工艺进行了优化，确定了SJ酸解产氢量最高的条件为：蔗糖10 g/L，酵母膏3 g/L，pH 6.0。与分批发酵相比，采用细胞循环的补料分批发酵在产氢率和产氢率方面显示出显著的提高。最大产率达到5.22 mmol-H2/L·h，比单批产率（1.73 mmol-H2/L·h）提高201.7%。最高产氢量为2.23 mol-H2/mol-己糖，比单批发酵提高65.2%。纤维床生物反应器（FBB）的细胞固定化也提高了氢气生产率，为游离细胞分批发酵的2.6倍。本研究证明了产乙醇菌产氢的可行性，通过在纤维床生物反应器中循环细胞和固定化细胞，获得了产氢的大幅度提高。
Abstract Inexpensive biomass is an ideal substrate for low-cost production of biohydrogen, but little was known about the biomass utilization performance of the ethanol-type hydrogen-producing bacteria Ethanoligenens harbinense. This study investigated the feasibility and performance of biohydrogen production by Ethanoligenens harbinense from sugarcane juice (SJ). Medium and process were firstly optimized with SJ as carbon source and the highest hydrogen yield was obtained when SJ was hydrolyzed by acid, and the medium contained 10 g/L sugar and 3 g/L yeast extract with pH at 6.0. Compared to batch fermentation, fed-batch fermentation with cell recycle showed significant improvement in hydrogen productivity and yield. The maximum productivity reached 5.22 mmol-H2/L•h, 201.7% higher than that of the single batch (1.73 mmol-H2/L•h). The highest H2 yield was 2.23 mol-H2/mol-hexose, 65.2% higher than that of single batch fermentation. Cell immobilization with the fibrous bed bioreactor (FBB) also improved hydrogen productivity to 2.6 times that of batch fermentation with free cells. This study demonstrated the feasibility of hydrogen production from SJ with Ethanoligens sp., and a great improvement in hydrogen production was obtained by recycling cells and immobilizing cells in a fibrous bed bioreactor.
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影响因子：
5
作者：
Jianzhi Zhang;Le Yu;Mengmeng Xu;Shangtian Yang;Q. Yan;Meng Lin;I. Tang
通讯作者：
Jianzhi Zhang;Le Yu;Mengmeng Xu;Shangtian Yang;Q. Yan;Meng Lin;I. Tang
影响因子：
4.4
作者：
M. Zwietering;I. Jongenburger;F. Rombouts;K. Riet
通讯作者：
M. Zwietering;I. Jongenburger;F. Rombouts;K. Riet
影响因子：
7.2
作者：
Sakchai Pattra;C. Lay;Chiu-Yue Lin;S. O-thong;A. Reungsang
通讯作者：
Sakchai Pattra;C. Lay;Chiu-Yue Lin;S. O-thong;A. Reungsang
影响因子：
11.4
作者：
Antonopoulou, Georgia;Gavala, Hariklia N.;Lyberatos, Gerasimos
通讯作者：
Lyberatos, Gerasimos
影响因子：
7.4
作者：
Ortigueira, Joana;Alves, Luis;Moura, Patricia
通讯作者：
Moura, Patricia