GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer.
GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer.
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GOLPH3 过表达与临床 N0 口腔舌癌患者的肿瘤进展和不良预后相关
DOI：
10.1186/1479-5876-10-168
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发表时间：
2012-08-20
影响因子：
7.4
通讯作者：
Song M
中科院分区：
文献类型：
--
作者：
Li H;Guo L;Chen SW;Zhao XH;Zhuang SM;Wang LP;Song LB;Song M
背景：GOLPH3（高尔基磷酸化蛋白3,34 kDa）的过度表达与许多实体瘤类型的进展相关，导致不良的临床结果。我们旨在探讨GOLPH3表达在临床N0 （cN0）口腔舌癌发生发展中的临床意义。方法采用real -time PCR和Western blotting技术检测4株口腔舌癌细胞系、原代培养的正常舌上皮细胞（TEC）、8对匹配的口腔舌癌样本和同一患者的癌旁非癌组织样本中GOLPH3的表达。采用免疫组化（IHC）方法检测了179例cN0口腔舌癌石蜡包埋组织中GOLPH3蛋白的表达。采用统计学方法评价GOLPH3表达与临床参数的相关性及诊断价值。结果与原代培养的正常舌上皮细胞（TEC）和邻近非癌组织样品相比，golph3 mRNA和蛋白在口腔舌癌细胞系和癌组织中表达上调。GOLPH3蛋白水平与临床分期（P= 0.001）、T分型（P= 0.001）、N分型（P= 0.043）、复发（P= 0.009）呈正相关。GOLPH3高表达患者总生存时间较短，而GOLPH3低表达患者总生存时间较长。结论GOLPH3过表达与cN0口舌癌患者预后不良相关，可能是一种新的、有用的预测cN0口舌癌预后的指标。
BackgroundOverexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. We aimed to investigate the clinical significance of GOLPH3 expression in the development and progression of clinically N0 (cN0) oral tongue cancer.MethodsReal-time PCR and Western blotting analyses were employed to examine GOLPH3 expression in four oral tongue cancer cell lines, primary cultured normal tongue epithelial cells (TEC), eight matched pairs of oral tongue cancer samples and adjacent noncancerous tissue samples from the same patient. Immunohistochemistry (IHC) was performed to examine GOLPH3 protein expression in paraffin-embedded tissues from 179 cN0 oral tongue cancer patients. Statistical analyses were applied to evaluate the diagnostic value and the associations of GOLPH3 expression with clinical parameters.ResultsGOLPH3 mRNA and protein was up-regulated in oral tongue cancer cell lines and cancerous tissues compared with that in primary cultured normal tongue epithelial cells (TEC) and adjacent noncancerous tissue samples. GOLPH3 protein level was positively correlated with clinical stage (P= 0.001), T classification (P= 0.001), N classification (P= 0.043) and recurrence (P= 0.009). Patients with higher GOLPH3 expression had shorter overall survival time, whereas those with lower GOLPH3 expression had longer survival time.ConclusionOur results suggest GOLPH3 overexpression is associated with poor prognosis for cN0 oral tongue cancer patients and may represent a novel and useful prognostic indicator for cN0 oral tongue cancer.
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影响因子：
168.9
作者：
Wang, YX;Klijn, JGM;Foekens, JA
通讯作者：
Foekens, JA
影响因子：
56.9
作者：
Tu, Linna;Tai, William C. S.;Banfield, David K.
通讯作者：
Banfield, David K.
影响因子：
4.5
作者：
Wu, CC;Taylor, RS;Howell, KE
通讯作者：
Howell, KE
影响因子：
64.8
作者：
Scott, Kenneth L.;Kabbarah, Omar;Liang, Mei-Chih;Ivanova, Elena;Anagnostou, Valsamo;Wu, Joyce;Dhakal, Sabin;Wu, Min;Chen, Shujuan;Feinberg, Tamar;Huang, Joseph;Saci, Abdel;Widlund, Hans R.;Fisher, David E.;Xiao, Yonghong;Rimm, David L.;Protopopov, Alexei;Wong, Kwok-Kin;Chin, Lynda
通讯作者：
Chin, Lynda
DOI：
10.1158/1078-0432.ccr-09-1695
发表时间：
2010-04-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Scott KL;Chin L
通讯作者：
Chin L