Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana.
Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana.
复制标题
AHL9过表达加速拟南芥叶片衰老。
DOI：
10.1186/s12870-022-03622-9
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发表时间：
2022-05-19
影响因子：
5.3
通讯作者：
Guo, Siyi
中科院分区：
文献类型：
--
作者：
Zhou, Yusen;Zhang, Xiaomin;Chen, Jing;Guo, Xiaopeng;Wang, Hongyan;Zhen, Weibo;Zhang, Junli;Hu, Zhubing;Zhang, Xuebing;Botella, Jose Ramon;Ito, Toshiro;Guo, Siyi
叶片衰老是叶片生长发育的最后阶段，受多种内在因素和环境信号的调控。乙烯是植物衰老的关键激素之一，但乙烯诱导叶片衰老的分子机制尚不清楚。在这项研究中，我们确定了一个AT-钩状（阿勒）蛋白，AHL 9，作为一个积极的调节叶片衰老拟南芥。AHL 9的过表达显著加速了与年龄相关的叶片衰老，并促进了黑暗诱导的叶片失绿。在AHL 9过表达系中观察到的早衰表型被乙烯生物合成抑制剂氨氧基乙酸抑制，表明乙烯参与AHL 9相关的衰老。RNA-seq和定量逆转录PCR（qRT-PCR）数据鉴定了在AHL 9过表达转基因植物的叶片中差异表达的许多衰老相关基因。我们的研究表明，AHL 9的功能，在加速叶片衰老过程中通过乙烯合成或信号。在线版本包含补充材料，可通过10.1186/s12870-022-03622-9获得。
Leaf senescence, the final stage of leaf growth and development, is regulated by numerous internal factors and environmental cues. Ethylene is one of the key senescence related hormones, but the underlying molecular mechanism of ethylene-induced leaf senescence remains poorly understood. In this study, we identified one AT-hook like (AHL) protein, AHL9, as a positive regulator of leaf senescence in Arabidopsis thaliana. Overexpression of AHL9 significantly accelerates age-related leaf senescence and promotes dark-induced leaf chlorosis. The early senescence phenotype observed in AHL9 overexpressing lines is inhibited by the ethylene biosynthesis inhibitor aminooxyacetic acid suggesting the involvement of ethylene in the AHL9-associated senescence. RNA-seq and quantitative reverse transcription PCR (qRT-PCR) data identified numerous senescence-associated genes differentially expressed in leaves of AHL9 overexpressing transgenic plants. Our investigation demonstrates that AHL9 functions in accelerating the leaf senescence process via ethylene synthesis or signalling. The online version contains supplementary material available at 10.1186/s12870-022-03622-9.
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