A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa.
A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa.
复制标题
膜结合 NAC 样转录因子 OsNTL5 抑制水稻开花
DOI：
10.3389/fpls.2018.00555
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Ito T
中科院分区：
文献类型：
--
作者：
Guo S;Dai S;Singh PK;Wang H;Wang Y;Tan JLH;Wee W;Ito T
尽管水稻具有短日照（SD）的特性，但与拟南芥等长日照植物一样，水稻（Oryza sativa）也有一个保守的光周期网络来控制开花。开花或抽穗是水稻的重要农艺性状。NAC转录因子是植物转录因子中最大的家族之一，具有高度保守性。然而，它们在开花或抽穗期的功能还不是很清楚。OsNTL 5在水稻发育叶片和幼穗中的优先表达表明了其在开花中的作用。为了检测其功能，产生了三个独立的构建体，一个在C端跨膜结构域中具有缺失（OsNTL 5 CXC），OsNTL 5 CXC与SRDX转录抑制子基序融合，OsNTL 5 CXC与泛素启动子下的VP 16激活结构域一起使用，以在水稻中分别产生过表达系OsNTL 5 CXC、OsNTL 5 CXC-SRDX和OsNTL 5 CXC-VP。0 sNTL 5 YGC-VP系表现出早花表型。与此相反，具有0 sNTL 5 p2C和0 sNTL 5 p2C-SRDX的植物显示出非常强的晚开花表型，表明0 sNTL 5作为转录抑制因子抑制开花。蛋白质亚细胞定位分析表明，OsNTL 5的N-末端部分定位于细胞核后，蛋白质从其跨膜结构域在C-末端切割，并作为TF功能。通过实时定量PCR分析，在OsNTL 5的过量表达系中，负责日长信号的开花基因如早抽穗期1（Ehd 1）、抽穗期3a（Hd 3a）和水稻开花位点T1（RFT 1）的表达显著改变。ChIP-qPCR和水稻原生质体检测结果表明，OsNTL 5直接与Ehd 1启动子结合，负调控Ehd 1的表达，表现出24 h SD周期的光周期表达模式。因此，NAC-like TF OsNTL 5作为Ehd 1的上游因子发挥转录抑制因子的作用，抑制水稻开花。
In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1.
登录
查看更多内容
影响因子：
4.5
作者：
Gao H;Zheng XM;Fei G;Chen J;Jin M;Ren Y;Wu W;Zhou K;Sheng P;Zhou F;Jiang L;Wang J;Zhang X;Guo X;Wang JL;Cheng Z;Wu C;Wang H;Wan JM
通讯作者：
Wan JM
OsMADS57 和 OsTB1 之间的相互作用通过 DWARF14 调节水稻分蘖
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.4
作者：
Kim, Sang-Gyu;Lee, Sangmin;Park, Chung-Mo
通讯作者：
Park, Chung-Mo
影响因子：
11.6
作者：
Ng, Sophia;Ivanova, Aneta;Giraud, Estelle
通讯作者：
Giraud, Estelle
影响因子：
64.8
作者：
Hayama, R;Yokoi, S;Shimamoto, K
通讯作者：
Shimamoto, K