Immunolocalization of extracellular matrix proteins and collagen synthesis in first‐trimester human decidua
Immunolocalization of extracellular matrix proteins and collagen synthesis in first‐trimester human decidua
复制标题
妊娠早期人类蜕膜中细胞外基质蛋白和胶原合成的免疫定位
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. Little
中科院分区：
文献类型：
--
作者：
L. Kisalus;J. Herr;C. Little
用亲和纯化的抗人I型、III型、IV型、V型胶原、层粘连蛋白和纤维连接蛋白抗体，用间接免疫荧光法检测了人早孕蜕膜的细胞外基质(ECM)。此外，利用中间丝蛋白、间质标志物Vimentin和上皮标志物角蛋白的抗体探讨了“间充质-上皮样”分类对分化蜕膜细胞的有效性。用~3H-Pro标记蜕膜组织，然后用胶原型特异性抗体免疫沉淀合成的蛋白质，检测细胞外基质成分的生物合成。免疫荧光显示蜕膜细胞包埋在广泛的I型和III型胶原网络中，胞浆内染色显示蜕膜细胞合成了这些胶原蛋白。IV型胶原和层粘连蛋白定位于分化的蜕膜细胞周围的外层，外周细胞质中有明显的荧光。免疫反应阳性的V型胶原与外膜和外基质密切相关。纤维连接蛋白定位于整个蜕膜细胞外基质和蜕膜细胞胞浆中的纤维状和点状结构。分化的蜕膜细胞保留了一个“间充质”中间丝细胞骨架，其中含有丰富的波形蛋白细丝，但角蛋白细丝很少。培养24小时后，从蜕膜外植体的培养液中免疫沉淀出I型、III型、V型和较小程度的IV型胶原，证实了人早孕蜕膜在体外合成和分泌这些胶原蛋白。
The extracellular matrix (ECM) of first‐trimester human decidua was examined with indirect immunofluorescence using affinity‐purified antibodies to human collagen types I, III, IV, V, laminin, and fibronectin. In addition, the validity of the classification “mesenchymal‐epithelioid” for differentiated decidual cells was addressed using antibodies to the intermediate filament proteins, vimentin, a mesenchymal marker, and keratin, an epithelial marker. Biosynthesis of extracellular matrix components was examined by radiolabeling of decidual explants in culture with 3H‐proline, followed by immunoprecipitations of synthesized proteins with collagen type‐specific antibodies. Immunofluorescence showed decidual cells are embedded in an extensive network of collagen types I and III, and intracytoplasmic staining suggested synthesis of these collagens by the decidual cells. Collagen type IV and laminin localized in the external lamina which surrounds the differentiated decidual cell, and some fluorescence was evident in the peripheral cytoplasm. Immunoreactive collagen type V was observed in close association with the external lamina and in the peridecidual matrix. Fibronectin localized throughout the decidual ECM and in fibrillar and punctuate patterns in the decidual cell cytoplasm. Differentiated decidual cells retained a “mesenchymal” intermediate filament cytoskeleton containing an abundance of vimentin filaments, but very few, if any, keratin filaments. Collagen types I, III, V, and to a lesser extent, IV, were immunoprecipitated from the medium of decidual explants after 24 hours of culture, demonstrating in vitro synthesis and secretion of these collagens by first trimester human decidua.
平滑肌细胞的胶原胞外骨架。
DOI：
10.1016/s0174-173x(81)80014-3
发表时间：
1981
期刊：
Collagen and related research
影响因子：
--
作者：
Gay,S;Martinez-Hernandez,A;Rhodes,RK;Miller,EJ
通讯作者：
Miller,EJ
在无血清培养基中培养的人妊娠早期蜕膜细胞的生长特征：催乳素、前列腺素和纤连蛋白的产生。
影响因子：
3.6
作者：
Hochner-Celnikier,D;Ron,M;Eldor,A;Segal,S;Palti,Z;Fuks,Z;Vlodavsky,I
通讯作者：
Vlodavsky,I
由 α 1(V) 链（B 链）组成的胶原蛋白分子：明显定位于胞外骨架中。
DOI：
10.1016/s0174-173x(80)80007-0
发表时间：
1981
期刊：
Collagen and related research
影响因子：
--
作者：
Gay,S;Rhodes,RK;Gay,RE;Miller,EJ
通讯作者：
Miller,EJ