Host-Induced Gene Silencing of a Multifunction Gene Sscnd1 Enhances Plant Resistance Against Sclerotinia sclerotiorum.
Host-Induced Gene Silencing of a Multifunction Gene Sscnd1 Enhances Plant Resistance Against Sclerotinia sclerotiorum.
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宿主诱导的多功能基因 Sscnd1 基因沉默增强植物对核盘菌的抗性
DOI：
10.3389/fmicb.2021.693334
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发表时间：
2021
影响因子：
5.2
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
Ding Y;Chen Y;Yan B;Liao H;Dong M;Meng X;Wan H;Qian W
核盘菌（Sclerotinia sclerotiorum）是一种毁灭性的坏死型真菌病原体，对世界范围内的作物生产具有重大的经济影响。稻瘟病菌附着胞特异性（MAS）蛋白被认为参与了稻瘟病菌附着胞的形成。Sscnd 1是一个MAS同源基因，在S.菌核敲低Sscnd 1基因的表达可显著降低S.在完整的油菜叶片上，它们的毒力部分恢复。Sscnd 1基因沉默的菌株表现出复合附着胞形成和细胞完整性的缺陷。烟草脆裂病毒（TRV）介导的宿主诱导基因沉默（HIGS）瞬时沉默Sscnd 1导致烟草疾病发展的显着减少。3个转HIGS基因拟南芥品系对S. Sclerotiorum和Sscnd 1表达降低。通过茎环qRT-PCR证实转基因HIGS拟南芥系中特异性Sscnd 1 siRNA的产生。本研究表明，复合附着胞相关基因Sscnd 1是S.通过在宿主植物中表达Sscnd 1的沉默构建体可以控制核盘菌茎腐病。
Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen and has a substantial economic impact on crop production worldwide. Magnaporthe appressoria-specific (MAS) proteins have been suggested to be involved in the appressorium formation in Magnaporthe oryzae. Sscnd1, an MAS homolog gene, is highly induced at the early infection stage of S. sclerotiorum. Knock-down the expression of Sscnd1 gene severely reduced the virulence of S. sclerotiorum on intact rapeseed leaves, and their virulence was partially restored on wounded leaves. The Sscnd1 gene-silenced strains exhibited a defect in compound appressorium formation and cell integrity. The instantaneous silencing of Sscnd1 by tobacco rattle virus (TRV)-mediated host-induced gene silencing (HIGS) resulted in a significant reduction in disease development in tobacco. Three transgenic HIGS Arabidopsis lines displayed high levels of resistance to S. sclerotiorum and decreased Sscnd1 expression. Production of specific Sscnd1 siRNA in transgenic HIGS Arabidopsis lines was confirmed by stem-loop qRT-PCR. This study revealed that the compound appressorium-related gene Sscnd1 is required for cell integrity and full virulence in S. sclerotiorum and that Sclerotinia stem rot can be controlled by expressing the silencing constructs of Sscnd1 in host plants.
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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