Effects of first dimension eluent composition in two-dimensional liquid chromatography.
Effects of first dimension eluent composition in two-dimensional liquid chromatography.
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DOI：
10.1016/j.chroma.2011.02.019
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发表时间：
2011-04-22
期刊：
影响因子：
--
通讯作者：
Carr PW
中科院分区：
文献类型：
--
作者：
Li X;Carr PW
全二维液相色谱（LCxLC）由于其非凡的分辨能力，在过去的几年中受到了极大的关注。这种技术的最大优点是可以在相对较短的时间内产生非常高的高峰容量。已经采用了许多方法来最大化LCxLC中的峰值容量。在这项工作中，我们调查的第一维移动的阶段的选择性的影响。LCxLC与一维LC（1DLC）相比具有几个潜在的优点，因为至少在反相LC中可以使用非常规溶剂。例如，在第一维中强烈吸收UV的溶剂在LCxLC中不是问题。这是因为UV检测器被放置在第二维柱之后，当第一维洗脱液的脉冲到达第二维柱时，它们在感兴趣的溶质之前很好地移动，因此根本不干扰溶质峰的检测。到目前为止，反相1DLC中使用最广泛的溶剂是甲醇和乙腈。然而，2DLC的“UV优势”允许我们使用UV活性溶剂，例如丙酮。我们比较他们的差分选择性乙腈分离的23吲哚乙酸的植物生物学的利益。我们还将它们应用于玉米种子提取物的分离，这是一个非常复杂的样品。在两个样品组中，移动的相组成可以是增加两个维度的正交性并因此增加LCxLC的有效峰容量的重要参数。
Comprehensive two-dimensional liquid chromatography (LCxLC) has received a great deal of attention during the past few years because of its extraordinary resolving power. The biggest advantage of this technique is that very high peak capacities can be generated in a relatively short time. Numerous approaches to maximize the peak capacity in LCxLC have been employed. In this work we investigate the impact of the first dimension mobile phase on selectivity. LCxLC has several potential advantages over one-dimensional LC (1DLC) in that unconventional solvents, at least in reversed-phase LC, can be used. For example, solvents which strongly adsorb in the UV in the first dimension are not problematic in LCxLC. This so because the UV detector is placed after the second dimensional column, as pulses of the first dimension eluent arrive at the second dimensional column, they elute well before the solutes of interest and therefore do not interfere at all with detection of solute peaks. So far, the most widely used solvents in reversed-phase 1DLC are methanol and acetonitrile. However, the “UV advantage” of 2DLC allows us to employ UV active solvents, such as acetone. We compare their differential selectivities to that of acetonitrile for the separation of 23 indole acetic acids of interest in plant biology. We also apply them to the separation of a maize seed extract, a very complex sample. In both sample sets, mobile phase composition can be an important parameter to increase the orthogonality of the two dimensions and thus, to increase the effective peak capacity of LCxLC.
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DOI：
10.1016/s0021-9673(01)88298-4
发表时间：
1983-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
GUIOCHON, G;BEAVER, LA;ZAKARIA, M
通讯作者：
ZAKARIA, M
影响因子：
4.1
作者：
Jiang, XL;van der Horst, A;Schoenmakers, PJ
通讯作者：
Schoenmakers, PJ
影响因子：
7.4
作者：
Murphy, RE;Schure, MR;Foley, JP
通讯作者：
Foley, JP
影响因子：
7.4
作者：
Gilar, M;Olivova, P;Gebler, JC
通讯作者：
Gebler, JC
影响因子：
1.3
作者：
Dugo, Paola;Fernandez, Maria del Mar Ramirez;Mondello, Luigi
通讯作者：
Mondello, Luigi