Integrated analysis of glycan and RNA in single cells.
Integrated analysis of glycan and RNA in single cells.
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单细胞中聚糖和RNA的综合分析
DOI：
10.1016/j.isci.2021.102882
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发表时间：
2021-08-20
期刊：
影响因子：
5.8
通讯作者：
Tateno H
中科院分区：
文献类型：
--
作者：
Minoshima F;Ozaki H;Odaka H;Tateno H
单细胞测序已成为剖析细胞异质性的不可或缺的技术，但从未应用于聚糖和RNA的同时分析。我们利用寡核苷酸标记的凝集素，首先通过测序建立了基于凝集素的单细胞聚糖谱分析方法（scGlycan - seq）。然后我们将scGlycan - seq与单细胞转录组谱分析相结合，用于单细胞中聚糖和RNA的联合分析（scGR - seq）。利用scGR - seq，我们在单细胞分辨率下分析了人类诱导多能干细胞（hiPSCs）分化为神经祖细胞前后的两种模式。RNA和聚糖的结合比它们其中任何一种都更清晰地区分了两种细胞类型。此外，对单细胞中聚糖和RNA模式的整合分析发现了已知和未知的对hiPSCs具有特异性且与神经分化相协调的凝集素。综上所述，我们证明了scGR - seq能够揭示聚糖在多细胞系统中的细胞异质性和生物学作用。
scGlycan - seq是为通过测序进行单细胞聚糖谱分析而开发的
scGR - seq是为单细胞中聚糖和RNA的联合分析而开发的
scGR - seq比其中任何一种都更清晰地区分了两种不同的细胞类型
scGR - seq鉴定了与多能性和神经分化相关的凝集素
分子生物学；分子结构；分子生物学实验方法；细胞生物学；细胞生物学的组织层面
Single-cell sequencing has emerged as an indispensable technology to dissect cellular heterogeneity but never been applied to the simultaneous analysis of glycan and RNA. Using oligonucleotide-labeled lectins, we first established lectin-based glycan profiling of single cells by sequencing (scGlycan-seq). We then combined the scGlycan-seq with single-cell transcriptome profiling for joint analysis of glycan and RNA in single cells (scGR-seq). Using scGR-seq, we analyzed the two modalities in human induced pluripotent stem cells (hiPSCs) before and after differentiation into neural progenitor cells at the single-cell resolution. The combination of RNA and glycan separated the two cell types clearer than either one of them. Furthermore, integrative analysis of glycan and RNA modalities in single cells found known and unknown lectins that were specific to hiPSCs and coordinated with neural differentiation. Taken together, we demonstrate that scGR-seq can reveal the cellular heterogeneity and biological roles of glycans across multicellular systems. scGlycan-seq was developed for single-cell glycan profiling by sequencing scGR-seq was developed for joint analysis of glycan and RNA in single cells scGR-seq separated the two distinct cell types clearer than either one of them scGR-seq identified lectins related to pluripotency and neural differentiation Molecular biology; Molecular Structure; Molecular biology experimental approach; Cell biology; Organizational aspects of cell biology
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影响因子：
16.6
作者：
Hayashi T;Ozaki H;Sasagawa Y;Umeda M;Danno H;Nikaido I
通讯作者：
Nikaido I
影响因子：
4
作者：
Kim, Dae-Kwan;Cha, Young;Park, Kyung-Soon
通讯作者：
Park, Kyung-Soon
影响因子：
5.8
作者：
Angerer, Philipp;Haghverdi, Laleh;Buettner, Florian
通讯作者：
Buettner, Florian
DOI：
10.1074/jbc.m109.068353
发表时间：
2010-02-19
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
North SJ;Huang HH;Sundaram S;Jang-Lee J;Etienne AT;Trollope A;Chalabi S;Dell A;Stanley P;Haslam SM
通讯作者：
Haslam SM
DOI：
10.1007/978-1-4939-9240-9_6
发表时间：
2019-01-01
期刊：
SINGLE CELL METHODS: SEQUENCING AND PROTEOMICS
影响因子：
--
作者：
Bageritz, Josephine;Raddi, Gianmarco
通讯作者：
Raddi, Gianmarco