Distribution of a novel 57 kDa intermediate filament (IF) protein in the nervous system
Distribution of a novel 57 kDa intermediate filament (IF) protein in the nervous system
复制标题
一种新型 57 kDa 中间丝 (IF) 蛋白在神经系统中的分布
作者：
L. Parysek;R. Goldman
在PC12细胞体外组装2个周期后获得中间丝（IF）的主要成分是一种57 kDa的蛋白，而不是波形蛋白（Parysek and Goldman, 1987）。利用57 kDa蛋白的抗血清，在IF制剂的免疫印迹和各种大鼠组织的免疫荧光上检测到一种具有相同分子量的交叉反应抗原。在舌头、小肠和肾上腺的3-4微米冷冻切片上进行免疫定位研究，发现神经束和细口径神经纤维有清晰的标记。肾上腺髓质的染色质细胞和神经节细胞也被标记。在神经系统中，坐骨神经和脊髓背根的小口径神经纤维以及背白柱和背根神经节中可见强烈的标记。在神经节细胞中，小尺寸神经节细胞被优先标记，而针对150和200 kDa神经丝三重体（NFT）成分的单克隆抗体被标记为大尺寸神经节细胞。在迄今为止用57 kDa抗血清检测的大脑区域中，有脑神经成分的标记和几个区域的细纤维的标记，包括小脑的颗粒层和脑干的皮质脊髓束。对于每个组织，相邻切片用vimentin或胶质纤维酸性蛋白抗体处理后显示的标记模式不同于57 kDa或NFT抗体。这些结果表明57 kDa的中频蛋白是神经中频成分。此外，这种蛋白质只分布在某些神经元元件中；这些因素可能由神经系统中一种尚未被认识的功能模式统一起来。
A 57 kDa protein, that is not vimentin, is the major component of intermediate filaments (IF) obtained after 2 cycles of in vitro assembly from PC12 cells (Parysek and Goldman, 1987). By use of an antiserum to the 57 kDa protein, a cross-reacting antigen (of identical molecular weight) was detected on immunoblots of IF preparations and by immunofluorescence of various rat tissues. Immunolocalization studies on 3–4 micron frozen sections of tongue, small intestine, and adrenal gland showed bright labeling of nerve bundles and fine-caliber nerve fibers. The chromaffin cells and ganglion cells of the adrenal medulla also were labeled. In the nervous system, intense labeling was seen in small-caliber nerve fibers in sciatic nerve and spinal cord dorsal roots, as well as in the dorsal white columns and dorsal root ganglia. Of the ganglion cells, preferential labeling was seen in small-sized ganglion cells, whereas a monoclonal antibody to the 150 and 200 kDa neurofilament triplet (NFT) components labeled the large-sized ganglion cells. In the areas of the brain thus far examined with 57 kDa antiserum, there was labeling of components of cranial nerves and labeling of thin fibers in several areas, including the granular layer of the cerebellum and the corticospinal tract in the brain stem. For each tissue, adjacent sections treated with vimentin or glial fibrillary acidic protein antibody revealed labeling patterns distinct from that seen with either the 57 kDa or NFT antibodies. These results indicate that the 57 kDa IF protein is a neural IF component. Furthermore, this protein is distributed in only certain neuronal elements; these elements may be unified by an as yet unrecognized pattern of function in the nervous system.
发育中的小鸡脊髓中的中间丝蛋白。
影响因子：
2.7
作者：
Tapscott,SJ;Bennett,GS;Toyama,Y;Kleinbart,F;Holtzer,H
通讯作者：
Holtzer,H
DOI：
10.1126/science.7444459
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Langford,LA;Coggeshall,RE
通讯作者：
Coggeshall,RE