Multicomponent Peptide Hydrogels as an Innovative Platform for Cell-Based Tissue Engineering in the Dental Pulp.
Multicomponent Peptide Hydrogels as an Innovative Platform for Cell-Based Tissue Engineering in the Dental Pulp.
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多组分肽水凝胶是牙浆中基于细胞的组织工程的创新平台。
DOI：
10.3390/pharmaceutics13101575
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发表时间：
2021-09-28
期刊：
影响因子：
5.4
通讯作者：
Lundy FT
中科院分区：
文献类型：
--
作者：
Afami ME;El Karim I;About I;Krasnodembskaya AD;Laverty G;Lundy FT
鉴于抗生素耐药性水平的提高，迫切需要纳米材料和新型生物制剂来控制细菌感染。迄今为止，商业上可获得的自组装肽水凝胶尚未被广泛研究其抑制与组织工程部位（如牙根管）相关的微生物的能力。在这项工作中，我们评估了牙髓干/基质细胞与市售的多组分肽水凝胶的生物相容性。我们还确定了水凝胶中牙髓干/基质细胞（DPSC）培养对生长因子/细胞因子表达的影响。此外，为了研究自组装肽水凝胶的新方面，我们确定了它们对口腔病原体金黄色葡萄球菌、粪肠球菌和核梭杆菌的抗菌活性。我们发现自组装肽水凝胶和具有粘附基序Arg-Gly-Asp （RGD）功能化的水凝胶与DPSCs具有生物相容性，并且与2d培养的细胞相比，3D水凝胶培养的细胞产生离散的分泌组。此外，我们发现可溶性肽和组装的水凝胶对口腔病原体具有抗菌作用。鉴于其对口腔病原体的抗菌活性，与牙髓干/基质细胞的生物相容性以及血管生成分泌组的增强，多组分肽水凝胶有望用于翻译。
In light of the increasing levels of antibiotic resistance, nanomaterials and novel biologics are urgently required to manage bacterial infections. To date, commercially available self-assembling peptide hydrogels have not been studied extensively for their ability to inhibit micro-organisms relevant to tissue engineering sites such as dental root canals. In this work, we assess the biocompatibility of dental pulp stem/stromal cells with commercially available multicomponent peptide hydrogels. We also determine the effects of dental pulp stem/stromal cell (DPSC) culture in hydrogels on growth factor/cytokine expression. Furthermore, to investigate novel aspects of self-assembling peptide hydrogels, we determine their antimicrobial activity against the oral pathogens Staphylococcus aureus, Enterococcus faecalis, and Fusobacterium nucleatum. We show that self-assembling peptide hydrogels and hydrogels functionalized with the adhesion motif Arg-Gly-Asp (RGD) are biocompatible with DPSCs, and that cells grown in 3D hydrogel cultures produce a discrete secretome compared with 2D-cultured cells. Furthermore, we show that soluble peptides and assembled hydrogels have antimicrobial effects against oral pathogens. Given their antibacterial activity against oral pathogens, biocompatibility with dental pulp stem/stromal cells and enhancement of an angiogenic secretome, multicomponent peptide hydrogels hold promise for translational use.
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影响因子：
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作者：
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通讯作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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